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Karyological and serological studies in Rana lessonae, R. ridibunda and in their hybrid R. ‘esculenta’ (Amphibia, Anura)

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Abstract

The karyotypes of the three water frog forms: Rana lessonae, R. ridibunda and R. ‘esculenta’ were analysed from bone marrow cell preparations of animals captured in several localities of the GDR. In the three forms chromosome morphology was similar (5 large and 8 small pairs), although differences in the relative length of most elements were found; R. ‘esculenta’ chromosomes were always intermediate.

One of the small pairs (Chr. 12) was found to be metacentric in R. lessonae and submetacentric in R. ridibunda. Most R. ‘esculenta’ individuals examined had one meta-and one submetacentric 12th element, indicating the hybrid nature of this form. However 16.6% ‘esculenta’ proved to be homozygous for either the metacentric or the submetacentric chromosome 12, while 13% lessonae individuals and 7.7% ridibunda were heterozygous for this element.

By starch gel electrophoresis an analysis was undertaken of serum proteins from water frogs coming from regions in which the forms occur together (sympatric populations) and from zones in which only one of them lives (allopatric populations).

In Rana lessonae, where only one allele had been previously described, two different alleles were found in animals coming from the GDR.

If genetic polymorphism is excluded, between 6.1% and 9.1% individuals from sympatric lessonae and ridibunda populations showed introgression of an albumin allele. No gene introgression was found in allopatric lessonae populations from the Leningrad region or in ridibunda from Alma Ata and southern Bulgaria.

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Authors and Affiliations

  1. Museum für Naturkunde, Humboldt Universität, Invalidenstr. 43, 104, Berlin, DDR

    S. Koref-Santibanez & R. Günther

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  1. S. Koref-Santibanez
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  2. R. Günther
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Koref-Santibanez, S., Günther, R. Karyological and serological studies in Rana lessonae, R. ridibunda and in their hybrid R. ‘esculenta’ (Amphibia, Anura). Genetica 52, 195–207 (1980). https://doi.org/10.1007/BF00121828

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