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A UV-spectroscopic study of the in vitro association of type I+III collagens with elastin solubilized peptides

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Abstract

The recently described in vitro association between elastin or elastin solubilized peptides (ESP) and type I+III collagens has been previously proved by scanning and transmission electron microscopy and by histological studies. The use of a UV-spectroscopic method corroborates the identity of the reaction and enable us to be more precise about its mechanism. The collagen-ESP association is characterized by a 224 nm peak absorbance; the kinetic at this wavelength shows the same three characteristic phases as observed by turbidimetry curves for collagen fibrillogenesis. Spectroscopic studies concerning the effect of Ca2+ on the reaction reveal that its presence decreases the lag phase and increases the speed of the growth phase; without Ca2+, collagen can self-react but with a smaller amplitude. Our investigations confirm that, when present, ESP plays a conclusive role in collagen organization.

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References

  1. H. K. Kleinman, L. Luckenbill-Edds, F. W. Cannon and G. C. Sephel, Anal. Biochem. 166 (1987) 1.

    Google Scholar 

  2. C. J. Doilon and F. H. Silver, Biomaterials 7 (1986) 3.

    Google Scholar 

  3. D. HAYEK, F. BERTHOD, O. DAMOUR and C. COLLOMBEL, Fourth Biomaterial Congress, Berlin (1992) Abstract, p. 582.

  4. E. Chignier, R. Eloy, A. Huc, R. Gimeno and C. Gleizal, J. Biomed. Mater. Res. 19 (1985) 115.

    Google Scholar 

  5. R. A. Gelman, B. R. Williams and K. A. Piez, J. Biol. Chem. 254 (1979) 180.

    Google Scholar 

  6. M. Rabaud, F. Lefebvre and D. Ducassou, Biomaterials 12 (1991) 313.

    Google Scholar 

  7. F. Lefebvre, S. Gorecki, R. Bareille, J. Amédée, L. Bordenave and M. Rabaud, Biomaterials 13 (1992) 28.

    Google Scholar 

  8. M. P. Jacob and W. Hornebeck, in “Frontiers of matrix biology”, edited by L. Robert (S. Karger, Basel, 1989) p. 92.

    Google Scholar 

  9. Authors of chapter, in “Handbook of biochemistry and molecular biology”, Vol. 1 Proteins, 3rd Edn, edited by G. D. Fasman (CRC Press, Boca Ratan, 1975) p. 193.

  10. J. Thomas, D. F. Elsden and S. M. Partridge, Nature 200 (1963) 651.

    Google Scholar 

  11. D. B. WESTLANFER, in “Advances in protein chemistry” (Academic Press, 1962) p. 303.

  12. T. R. Oegema, J. Laidlaw, V. C. Hascall and D. D. Dziewiatkowski, Arch. Biochem. Biophys. 170 (1975) 698.

    Google Scholar 

  13. N. P. Ward, D. J. S. Hulmes and J. A. Chapman, J. Mol. Biol. 190 (1986) 107.

    Google Scholar 

  14. P. Mould, D. J. S. Hulmes, D. F. Holmes, C. Cummings, C. H. J. Sear and J. A. Chapman, J. Mol. Biol. 211 (1990) 581.

    Google Scholar 

  15. D. W. Urry, Perspect. Biol. Med. 18 (1974) 68.

    Google Scholar 

  16. L. Bordenave, F. Lefebvre, R. Bareille, F. Rouais, Ch. Baquey and M. Rabaud, Biomaterials 13 (1992) 439.

    Google Scholar 

  17. N. Bonzon, P. Picard, F. Lefebvre, F. Rouais and M. Rabaud, ITBM, 14 (1993) 39.

    Google Scholar 

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Authors and Affiliations

  1. IBCN-CNRS, Université de Bordeaux II-146, rue Léo Saignat, 33076, Bordeaux Cédex, France

    M. Hernould & P. V. Graves

  2. U. 306-INSERM, Université de Bordeaux II-146, rue Léo Saignat, 33076, Bordeaux Cédex, France

    N. Bonzon & M. Rabaud

Authors
  1. M. Hernould
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  2. N. Bonzon
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  3. M. Rabaud
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  4. P. V. Graves
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Hernould, M., Bonzon, N., Rabaud, M. et al. A UV-spectroscopic study of the in vitro association of type I+III collagens with elastin solubilized peptides. J Mater Sci: Mater Med 6, 24–28 (1995). https://doi.org/10.1007/BF00121242

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  • DOI: https://doi.org/10.1007/BF00121242

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