Abstract
Somatic embryos were obtained from young, isolated cotyledons of Picea abies. A bud-inductive treatment for one week before transfer to a callus induction medium, resulted in a tenfold increase in the percentage of cotyledons in which embryonic tissues could be induced. The degree of expression of somatic embryogeny depends on whether cotyledons are first subjected to a callus-inducing medium or first exposed to a cytokinin treatment followed by a callus-inducing medium. It is hypothesized that two populations of cells exist, both with the inherent potential of becoming embryogenic after treatment with plant growth regulators.
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Lelu, MA.P., Boulay, M.P. & Borivman, C.H. Somatic embryogenesis in cotyledons of Picea abies is enhanced by an adventitious bud-inducing treatment. New Forest 4, 125–135 (1990). https://doi.org/10.1007/BF00119005
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DOI: https://doi.org/10.1007/BF00119005