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Colchicine action at meiotic prophase revealed by SC-spreading

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Abstract

In the present paper earlier findings on the interference of colchicine with presynaptic alignment and synaptonemal complex formation in Allium ursinum are corroborated and several other direct or indirect consequences of colchicine treatment, like deformations and decomposition of lateral elements and a high incidence of non-homologous associations are reported. Mechanisms of meiotic alignment and pairing are discussed with respect to their susceptibility to the known cytological and biochemical activities of colchicine.

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References

  • Albini, S. M. & Jones, G. H., 1984. Synaptonemal complex associated centromeres and recombination nodules in plant meiocytes prepared by an improved surface-spreading technique. Expl Cell Res. 155: 588–592.

    Google Scholar 

  • Albini, S. M. & Jones, G. H., 1987. Synaptonemal complex spreading in Allium cepa and A. fistulosum. I. The initiation and sequence of pairing. Chromosoma 95: 324–338.

    Google Scholar 

  • Ashley, T., 1978. Effect of colchicine on somatic crossing over induced by mitomycin C in soybean (Glycine max). Genetica 49: 87–96.

    Google Scholar 

  • Avivi, L., 1976. Colchicine induced bivalent pairing of tetraploid microsporocytes in Triticum longissimum and T. speltoides. Can. J. Genet. Cytol. 18: 731–738.

    Google Scholar 

  • Avivi, L., Feldman, M. & Bushuk, W., 1969. The mechanism of somatic association in common wheat, Triticum aestivum L. I. Suppression of somatic association by colchicine. Genetics 62: 745–752.

    Google Scholar 

  • Barber, H. N., 1942. The experimental control of chromosome pairing in Fritillaria. J. Genet. 43: 359–374.

    Google Scholar 

  • Bennett, M. D. & Smith, J. B., 1979. The effect of colchicine on fibrillar material in wheat meiocytes. J. Cell Sci. 38: 33–47.

    Google Scholar 

  • Bennett, M. D., Stern, H. & Woodward, M., 1974. Chromatin attachment to nuclear membrane of wheat pollen mother cells. Nature 252: 395–396.

    Google Scholar 

  • Bennett, M. D., Toledo, L. A. & Stern, H., 1979. The effect of colchicine on meiosis in Lilium speciosum cv. ‘Rosemede’. Chromosoma 72: 175–189.

    Google Scholar 

  • Dermen, H., 1938. A cytological analysis of polyploidy induced by colchicine and by extremes of temperature. J. Heredity 29: 211–229.

    Google Scholar 

  • Dover, G. A. & Riley, R., 1973. The effect of spindle inhibitors applied before meiosis on meiotic chromosome pairing. J. Cell Sci. 12: 143–161.

    Google Scholar 

  • Dover, G. A. & Riley, R., 1977. Inferences from genetical evidence on the course of meiotic chromosome pairing in plants. Phil. Trans. Roy. Soc. Lond. B 277: 313–326.

    Google Scholar 

  • Driscoll, C. J. & Darvey, N. L., 1970. Chromosome pairing: effect of colchicine on an isochromosome. Science 169: 290–291.

    Google Scholar 

  • Driscoll, C. J., Darvey, N. L. & Barber, H. N., 1967. Effect of colchicine on meiosis of hexaploid wheat. Nature 216: 687–688.

    Google Scholar 

  • Feldman, M. & Avivi, L., 1988. Genetic control of bivalent pairing in common wheat: the mode of Ph1 action. In: Kew Chromos. Conf. III (P. E.Brandham, ed.), pp. 269–279. London, HMSO.

    Google Scholar 

  • Gillies, C. B., 1984. The synaptonemal complex in higher plants. CRC Crit. Rev. Plant Sci. 2/2: 81–116.

    Google Scholar 

  • Gillies, C. B., 1985. An electron microscopic study of synaptonemal complex formation at zygotene in rye. Chromosoma 92: 165–175.

    Google Scholar 

  • Hasenkampf, C. A., 1984. Synaptonemal complex formation in pollen mother cells of Tradescantia. Chromosoma 90: 275–284.

    Google Scholar 

  • Heslop-Harrison, J. S., Smith, J. B. & Bennett, M. D., 1988. The absence of the somatic association of centromeres of homologous chromosomes in grass mitotic metaphases. Chromosoma 96: 119–131.

    Google Scholar 

  • Hotta, Y. & Shepard, J. Biochemical aspects of colchicine action on meiotic cells. Mol. gen. Genet. 122: 243–260.

  • Jackson, R. C. & Murray, B. G., 1983. Colchicine induced quadrivalent formation in Helianthus: evidence of ancient polyploidy. Theor. appl. Genet. 64: 219–222.

    Google Scholar 

  • Levan, A., 1939. The effect of colchicine on meiosis in Allium. Hereditas 25: 9–26.

    Google Scholar 

  • Lin, Y. J., 1982. Temperature and chiasma formation in Rhoeo spathacea var. variegata. Genetica 60: 25–30.

    Google Scholar 

  • Loidl, J., 1987. Synaptonemal complex spreading in Allium ursinum: pericentric asynapsis and axial thickenings. J. Cell Sci. 87: 439–448.

    Google Scholar 

  • Loidl, J., 1988a. The effect of colchicine on synaptonemal complex formation in Allium ursinum. Expl Cell Res. 178: 93–97

    Google Scholar 

  • Loidl, J., 1988b. SC-formation in some Allium species, and a discussion of the significance of SC-associated structures and of the mechanism for presynaptic alignment. Pl. Syst. Evol. 158: 117–131.

    Google Scholar 

  • Loidl, J. & Jones, G. H., 1986. Synaptonemal complex spreading in Allium. I. Triploid A. sphaerocephalon. Chromosoma 93: 420–428.

    Google Scholar 

  • Murray, B. G., Jackson, R. C. & Barker, N. R. N., 1983. Cryptic genome homologies revealed by pre-meiotic colchicine treatment of meiocytes. In: Kew Chromos. Conf. II (P. E.Brandham & M. D.Bennett, eds), pp. 165–171. London, George Allen & Unwin.

    Google Scholar 

  • Parchman, L. G. & Stern, H., 1969. The inhibition of protein synthesis in meiotic cells and its effect on chromosome behavior. Chromosoma 26: 298–311.

    Google Scholar 

  • Puertas, M. J., de laPeña, A., Estades, B. & Merino, F., 1984. Early sensitivity to colchicine in developing anthers of rye. Chromosoma 89: 121–126.

    Google Scholar 

  • Rasmussen, S. W. & Holm, P. B., 1980. Mechanics of meiosis. Hereditas 93: 187–216.

    Google Scholar 

  • Salonen, K., Paranko, J. & Parvinen, M., 1982. A colcemid-sensitive mechanism involved in regulation of chromosome movements during meiotic pairing. Chromosoma 95: 611–618.

    Google Scholar 

  • Sheldon, J., Willson, C. & Dickinson, H. G., 1988. Interaction between the nucleus and cytoskeleton during the pairing stages of male meiosis in flowering plants. In: Kew Chromos. Conf. III (P. E.Brandham, ed.), pp. 27–35. London, HMSO.

    Google Scholar 

  • Shepard, J., Boothroyd, E. R. & Stern, H., 1974. The effect of colchicine on synapsis and chiasma formation in microsporocytes of Lilium. Chromosoma 44: 423–437.

    Google Scholar 

  • Sperling, K. & Lüdtke, E.-K., 1981. Arrangement of prematurely condensed chromosomes in cultured cells and lymphocytes of the Indian muntjac. Chromosoma 83: 541–553.

    Google Scholar 

  • Stadler, J. & Franke, W. W., 1972. Colchicine-binding proteins in chromatin and membranes. Nature New Biol. 237: 237–238.

    Google Scholar 

  • Steinegger, E. & Levan, A., 1948. The C-mitotic qualities of colchicine, trimethyl colchicinic acid and two phenanthrene derivatives. Hereditas 34: 193–203.

    Google Scholar 

  • Therman, E. & Denniston, C., 1984. Random arrangement of chromosomes in Uvularia (Liliaceae). Pl. Syst. Evol. 147: 289–297.

    Google Scholar 

  • Thomas, J. B. & Kaltsikes, P. J., 1977. The effect of colchicine on chromosome pairing. Can. J. Genet. Cytol. 19: 231–249.

    Google Scholar 

  • Toledo, L. A., Bennett, M. D. & Stern, H., 1979. Cytological investigations of the effect of colchicine on meiosis in Lilium hybrid cv. ‘Black Beauty’ microsporocytes. Chromosoma 72: 157–173.

    Google Scholar 

  • Walker, R. I., 1938. The effect of colchicine on microspore mother cells and microspores of Tradescantia paludosa. Amer. J. Bot. 25: 280–285.

    Google Scholar 

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  1. Institut für Botanik der Universität, Rennweg 14, A-1030, Wien, Austria

    J. Loidl

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Loidl, J. Colchicine action at meiotic prophase revealed by SC-spreading. Genetica 78, 195–203 (1988). https://doi.org/10.1007/BF00055638

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  • DOI: https://doi.org/10.1007/BF00055638

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