Abstract
The inorganic formulations of fourteen common plant tissue culture basal media were examined from the primary literature. Inaccuracies and errors were found for molecular formulae, chemical hydrations, and molar equivalences for iron/EDTA complexation. A comparison with published basal medium formulations from six commercial suppliers uncovered additional inaccuracies, modifications, and errors, thereby emphasizing the need for investigators to examine and describe medium formulations precisely in future publications.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson WC (1980) Tissue culture propagation of red and black raspberries, Rubus idaeus and R. occidentalis. Acta Hort. 112: 13–20
Anderson WC (1983) Micropropagation of filberts, Corylus avellana. Comb. Proc. Intern. Plant Prop. Soc. 33: 132–137 (Publ. 1984)
Blaydes DF (1966) Interaction of kinetin and various inhibitors in the growth of soybean tissue. Physiol. Plant. 19: 748–753
Boll WG & Street HE (1951) Studies on the growth of excised roots. I. The stimulatory effect of molybdenum and copper on the growth of excised tomato roots. New Phytol. 50: 52–75
Chu C-C (1978) The N6 medium and its applications to anther culture of cereal crops. In: Proc. Symp. on Plant Tiss. Cult. Science Press, Peking, pp 43–50
Chu C-C, Wang C-C, Sun C-S, Hsu C, Yin K-C, Chu C-Y & Bi F-Y (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scientia Sinica 18: 659–668
Eriksson T (1965) Studies on the growth requirements and growth measurements of cell cultures of Haplopappus gracilis. Physiol. Plant. 18: 976–993
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50: 151–158
Gamborg OL, Murashige T, Thorpe TA & Vasil IK (1976) Plant Tissue Culture Media. In Vitro 12: 473–478
Gresshoff PM & Doy CH (1974) Derivation of a haploid cell line from Vitis vinifera and the importance of the stage of meiotic development of anthers for haploid culture of this and other genera. Z. Pflanzenphysiol. 73: 132–141
Knudson L (1946) A new nutrient solution for the germination of orchid seed. Amer. Orchid Soc. Bull. 15: 214–217
Lloyd G & McCown B (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Intern. Plant Prop. Soc. Proc. 30: 421–427 (Publ. 1981)
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nagata T & Takebe I (1971) Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta 99: 12–20
Nitsch JP & Nitsch C (1969) Haploid plants from pollen grains. Science 163: 85–87
Schenk RU & Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204
Singh M & Krikorian AD (1980) Chelated iron in culture media. Ann. Bot. 46: 807–809
Singh M & Krikorian AD (1981) White's standard nutrient solution. Ann. Bot. 47: 133–139
Smith MAL & McCown BH (1982/83) A comparison of source tissue for protoplast isolation from three woody plant species. Plant Sci. Letters 28: 149–156
Vacin EF & Went FW (1949) Some pH changes in nutrient solutions. Botanical Gaz. 110: 605–613
White PR (1943) A Handbook of Plant Tissue Culture. The Jaques Cattell Press, Lancaster, Penn, 277 p
White PR (1963) The cultivation of animal and plant cells. Ronald Press Co, New York, 228 p
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Owen, H.R., Miller, A.R. An examination and correction of plant tissue culture basal medium formulations. Plant Cell Tiss Organ Cult 28, 147–150 (1992). https://doi.org/10.1007/BF00055509
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00055509