Abstract
Feulgen-DNA values and nuclear areas were evaluated microspectrophotometrically for epithelial cells of the ovarian follicle during oogenesis in Chrysomya putoria. The aim was to investigate whether polyploidization occurred in the cells surrounding the nurse chamber and/or in those around the oocyte as well as whether different DNA amounts were found regarding the cell types considered. Four DNA endoreduplicative cycles could be demonstrated for the epithelial cells regardless of their localization on the follicle, during oogenesis. A small percentage of epithelial cells reaches a 32C degree. The nuclear area, however, did not increase at the same rate in cells covering the oocyte as in those covering the nurse chamber, in some of the oogenesis stages. The meaning of endopolyploidy for these cells is discussed, considering reports on relevance of secretory activities and their maintenance in polyploid cell systems.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Avancini, R. M. P. & Prado, A. P., 1986. Oogenesis in Chrysomya putoria (Wiedemann) (Diptera: Calliphoridae). Int. J. Insect Morphol. Embryol. 15 (5/6): 375–384.
Brennan, M. D., Weiner, A. J., Goralski, T. J. & Mahowald, A. P., 1982. The follicle cells are a major site of vitellogenin synthesis in Drosophila melanogaster. Devel. Biol. 89: 225–236.
Cardoen, J., Schoofs, L., Broekaert, D., Van, Mellaert, H., Verachtert, B. & De, Loof, A., 1986. Polyploidization and localization of poly (A)+RNA in the different cell types of the vitellogenic meroistic ovary of the flesh fly, Sarcophaga bullata. Histochemistry 85: 305–312.
Geysen, J., Cardoen, J. & De, Loof, A., 1987. Distribution of yolk polypeptide in the follicle cells during the differentiation of the follicular epithelium in Sarcophasa bullata egg follicles. Development 101: 33–43.
Huebner, E., 1984. The ultrastructure and development of the telotrophic ovary. In: King, R. C. & Akai, H. (eds). Insect ultrastructure. vol. 2, Plenum Press, New York, p. 3–48.
King, R. C. & Vanoucek, E. G., 1960. Oogenesis in adult Drosophila melanogaster. X. Studies on the behavior of the follicle cells. Growth 24: 333–338.
Mulligan, P. K., & Rasch, E. M., 1985. Determination of DNA content in the nurse and follicle cells from wild type and mutant Drosophila melanogaster by DNA-Feulgen cytophotometry. Histochemistry 82: 233–247.
Nagl, W., 1978. Endopolyploidy and polyteny in differentiation and evolution. Amsterdam, North-Holland Publ. Co.
Palkóvits, M. & Fisher, J., 1968. Karyometric investigations. Budapest, Akademiai Kiadó.
Rubacha, A., Tucker, M. A., De, Valoir, R., Belikoff, E. J. & Beckingham, K., 1988. Genes with specific functions in the ovarian follicles of Calliphora erythrocepala (Diptera). Devel. Biol. 129: 449–463.
Telfer, W. H., Huebner, E. & Smith, D. S., 1982. The celi biology of vitellogenic follicles in Hyalophora and Rhodnius. In: King, R. E. & Akai, N. (eds.) Insect ultrastructure, Vol. I, Plenum Press, New York, p. 118–149
Woodruff, R. I., Huebner, E. & Telfer, W. H., 1986. Ion currents in Hyalophora ovaries: the role of the epithelium and the intercellular spaces of the trophic cap. Devel. Biol. 117: 405–416.
Zeiss Informatin, 1977. Continuous filter monochromator b with motor drive. Mikro Bull. 18: 1–4.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Avancini, R.M.P., Mello, M.L.S. Endopolyploidy in follicle epithelial cells around the nurse chamber during oogenesis in Chrysomya putoria (Diptera, Calliphoridae). Genetica 81, 5–10 (1990). https://doi.org/10.1007/BF00055230
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00055230