Abstract
The effect of GA3 on somatic embryogenesis from petiole fragments excised from micropropagated fennel plantlets was studied. Explants were maintained for 4 weeks on an induction medium containing, 2,4-d and kinetin and were then transferred to a medium devoid of these growth regulators to allow embryo development. The addition of autoclaved or filter-sterilized GA3 to the induction medium or to the embryo development medium increased the number of embryogenic explants. No positive effect was observed when GA3 was added to the micropropagation medium of the mother plantlets. Gibberellic acid also counteracted the inhibiting effect of continuous light on the number of embryogenic explants. Moreover, the embryogenic frequency of petiole explants from several genotypes previously considered as poorly reacting was highly enhanced by GA3.
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Abbreviations
- BA:
-
6-benzyladenine
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- GA3 :
-
Gibberellic acid
- IAA:
-
Indole-3-acetic acid
- IBA:
-
Indole-3-butyric acid
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Hunault, G., Maatar, A. Enhancement of somatic embryogenesis frequency by gibberellic acid in fennel. Plant Cell Tiss Organ Cult 41, 171–176 (1995). https://doi.org/10.1007/BF00051587
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DOI: https://doi.org/10.1007/BF00051587