Abstract
Shoots of Miscanthus X ogiformis Honda ‘Giganteus’ transferred from medium with benzyladenine (BA) to thidiazuron (TDZ) formed significantly more axillary shoots than shoots grown continuously on either medium, or transferred from TDZ to BA. Shoot formation on axillary shoots transferred from BA-containing media to media with kinetin or isopentenyladenine (2iP) or transferred from media with TDZ, kinetin or 2iP to media with BA, corresponded to the number of shoots formed in the previous subculture. Shoot formation on shoots transferred from medium containing BA to media containing combinations of BA and/or TDZ increased with increasing concentrations of TDZ in the first subculture, whereas shoot formation in the second and third subculture depended on the BA concentration. When shoots were transferred from media with BA to media with TDZ, the time for shoot formation, as well as shoot size, indicate that the combined effect of BA and TDZ is expressed only during the early phase of the subculture. The results suggest that adenine- and phenylurea-type cytokinins have a common binding site in the plant cell, and a model is proposed.
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Abbreviations
- BA:
-
6-benzyladenine
- CBP:
-
cytokinin-binding protein
- 2iP:
-
isopentenyladenine
- KIN:
-
kinetin
- MS:
-
Murashige & Skoog
- TDZ:
-
thidiazuron
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Nielsen, J.M., Hansen, J. & Brandt, K. Synergism of thidiazuron and benzyladenine in axillary shoot formation depends on sequence of application in Miscanthus X ogiformis ‘Giganteus’. Plant Cell Tiss Organ Cult 41, 165–170 (1995). https://doi.org/10.1007/BF00051586
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DOI: https://doi.org/10.1007/BF00051586