Abstract
Supplementation of MS-based medium containing 4.4 μM 6-benzyladenine and 2.7 μM α-naphthaleneacetic acid with 0.001–0.1% (w/v) of the non-ionic, co-polymer surfactant, Pluronic F-68, significantly (p<0.05) increased the mean fresh weight gain of cultured leaf explants of chrysanthemum (Dendranthema grandiflora) “Tone Maid” and “Early Charm” by a maximum of 74% and 34%, respectively. The percentage of individual explants giving adventive shoots was also stimulated by Pluronic F-68; for cv. Early Charm, 0.001% (w/v) Pluronic F-68 induced the maximum response, whereas for cv. Tone Maid, the maximum response occurred with 0.01% surfactant. Shoot regeneration from explants was also enhanced at these concentrations of surfactant, compared to explants cultured in the absence of Pluronic F-68.
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Abbreviations
- BA:
-
6-benzyladenine
- MS:
-
Murashige & Skoog 1962
- NAA:
-
α-naphthaleneacetic acid
References
Anderson N O (1987) Reclassifications of the genus Chrysanthemum L. Hort. Science 22: 313
Broertjes C, Koene P & van Heen J W H (1980) A mutant of a mutant of a mutant of a ...: irradiation of progressive radiation-induced mutants in a mutation-breeding programme with Chrysanthemum morifolium Ram. Euphytica 29: 525–530
Khatun A, Laouar L, Davey M R, Power J B, Mulligan B J & Lowe K C (1993) Effects of Pluronic F-68 on shoot regeneration from cultured jute cotyledons and on growth of transformed roots. Plant Cell Tiss. Org. Cult. 34: 133–140
Kumar V, Laouar L, Davey M R, Mulligan B J & Lowe K C (1992) Pluronic F-68 stimulates growth of Solanum dulcamara in culture. J. Exp. Bot. 43: 487–492
Lindsay G C & Ledger S E (1993) A protoplast to plant system for the chrysanthemum Dendranthema zawadskii x D. grandiflora. Plant Cell Rep. 12: 278–280
Lowe J M, Davey M R, Power J B & Blundy K S (1993a) A study of some factors affecting Agrobacterium transformation and plant regeneration of Dendranthema grandiflora Tzvelev (syn. Chrysanthemum morifolium Ramat.). Plant Cell Tiss. Org. Cult. 33: 171–180
Lowe K C, Davey M R, Power J B & Mulligan B J (1993b) Surfactant supplements in plant culture systems. Agro-food Ind. Hi-Tech 4: 9–13
Malaure R S, Barclay G, Power J B & Davey M R (1991a) The production of novel plants from florets of Chrysanthemum morifolium using tissue culture. 1. Shoot regeneration from ray florets and somaclonal variation exhibited by the regenerated plants. J. Plant Physiol. 139: 8–13
Malaure R S, Barclay G, Power J B & Davey M R (1991b) The production of novel plants from florets of Chrysanthemum morifolium using tissue culture. 2. Securing natural mutations (sports). J. Plant Physiol. 139: 14–18
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
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Khehra, M., Lowe, K.C., Davey, M.R. et al. An improved micropropagation system for Chrysanthemum based on Pluronic F-68-supplemented media. Plant Cell Tiss Organ Cult 41, 87–90 (1995). https://doi.org/10.1007/BF00051576
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DOI: https://doi.org/10.1007/BF00051576