Abstract
Activity of extracellular phosphatases (phosphomonoesterases) was measured in sandy streambed sediments of the Breitenbach, a small unpolluted upland stream in Central Germany. Fluorigenic 4-methylumbelliferyl phosphate served as a model substrate. Experiments were conducted using sediment cores in a laboratory simulation of diffuse groundwater discharge through the stream bed, a natural process occurring in the Breitenbach as well as many other streams.
Streambed sediments contained high levels of particulate phosphorus, but concentrations of dissolved phosphorus in the interstitial water were 3 to 4 orders of magnitude lower. These interstitial concentrations were similar to those in the stream and groundwater. Extracellular phosphatase activity was high in the streambed sediments. These enzymes probably contribute significantly to the flux of phosphorus in sediment by hydrolyzing phosphomonoesters, making free phosphate available to the sediment microorganisms.
Factors influencing the kinetic parameters V max (maximum activity) and apparent K m (enzyme affinity) of phosphatase were discharge rates of water through the sediment, water quality (ground- or stream water), and substrate (phosphomonoesters) as well as dissolved ortho-phosphate concentrations. Enzymes are supposed to be effective at limiting substrate concentrations, where, in this study, changes in discharge rates had little influence on rates of hydrolysis. Higher V max and lower K m values were found during percolation of groundwater through the sediment cores, compared with stream water. This indicates that rates of hydrolysis were higher with groundwater, both at substrate limitation and at substrate saturation. This was probably a consequence of the lower levels of dissolved ortho-phosphate in the groundwater.
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References
Azam, F. & R. E. Hodson, 1981. Multiphasic kinetics for D-glucose uptake by assemblages of natural marine bacteria. Mar. Ecol. Prog. Ser. 6: 213–222.
Brehm, J. & M. P. D. Meijering, 1982. Fließgewässerkunde. Quelle & Meyer, Heidelberg, 311 pp. 165
Chrost, R. J. & J. Overbeck, 1987. Kinetics of alkaline phosphatase activity and phosphorus availability for phytoplankton and bacterioplankton in Lake Plußsee (North German eutrophic lake). Microb. Ecol. 13: 229–248.
Chrost, R. J., U. Münster, H. Rai, D. Albrecht, K.-P. Witzel & J. Overbeck, 1989. Photosynthetic production and exoenzymatic degradation of organic matter in the euphotic zone of a eutrophic lake. J. Plankton Res. 11: 223–242.
Cox, E., 1990. Studies on the algae of a small softwater stream. II. Algal standing crop (measured by chlorophyll-a) on soft and hard substrata. Arch. Hydrobiol./ Suppl. 83: 553–566.
Fiebig, D. M., 1992. Fates of dissolved free amino acids in groundwater discharged through stream-bed sediments. In B. T. Hart & P. G. Sly (eds), Sediment/Water Interactions. Developments in Hydrobiology 75. Kluwer Academic Publishers, Dordrecht: 311–319. Reprinted from Hydrobiologia 235/236.
Jansson, M., H. Olsson & O. Broberg, 1981. Characterization of acid phosphatases in the acidified Lake Gardsjön, Sweden. Arch. Hydrobiol. 92: 377–395.
Jansson, M., H. Olsson & K. Pettersson, 1988. Phosphatases; origin, characteristics and function in lakes. In G. Persson & M. Jansson (eds), Phosphorus in Freshwater Ecosystems. Developments in Hydrobiology 48. Kluwer Academic Publishers, Dordrecht: 157–175. Reprinted from Hydrobiologia 170.
Krambeck, C., 1979. Applicability and limitations of the Michaelis-Menten equation in microbial ecology. Arch. Hydrobiol. Beih. Ergebn. Limnol. 12: 64–76.
Leatherbarrow, R. J., 1987. Enzfitter. A non-linear regression data analysis program for the IBM PC/PS2. Biosoft, Cambridge, UK.
Marquardt, D. W., 1963. An algorithm for least-squares estimation of nonlinear parameters. J. Soc. ind. appl. Math. 11: 431–441.
Marxsen, J., 1980a. Untersuchungen zur Ökologie der Bakterien in der fließenden Welle von Bächen. I. Chemismus, Primärproduktion, CO2-Dunkelfixierung und Eintrag von partikulärem organischen Material. Arch. Hydrobiol./ Suppl. 57: 461–533.
Marxsen, J., 1980b. Untersuchungen zur Ökologie der Bakterien in der fließenden Welle von Bächen. III. Aufnahme gelöster organischer Substanzen. Arch. Hydrobiol./ Suppl. 58: 207–272.
Marxsen, J., 1988. Evaluation of the importance of bacteria in the carbon flow of a small open grassland stream, the Breitenbach. Arch. Hydrobiol. 111: 339–350.
Marxsen, J. & K. Moaledj, 1988. On the structure of bacterial communities in a Central European open grassland stream, the Breitenbach. Verh. int. Ver. Limnol. 23: 1855–1860.
Marxsen, J. & K. -P. Witzel, 1990. Measurement of exoenzymatic activity in streambed sediments using methylumbelliferyl-substrates. Arch. Hydrobiol. Beih. Ergebn. Limnol. 34: 21–28.
Marxsen, J. & K. -P. Witzel, 1991. Significance of extracellular enzymes for organic matter degradation and nutrient regeneration in small streams. In R. J. Chrost (ed.), Microbial Enzymes in Aquatic Environments. Brock/Springer Series in Contemporary Bioscience. Springer-Verlag, New York: 270–285.
Olsson, H., 1991. Phosphatase activity in an acid, limed Swedish lake. In R. J. Chrost (ed.), Microbial Enzymes in Aquatic Environments. Brock/Springer Series in Contemporary Bioscience. Springer-Verlag, New York: 206–219.
Overbeck, J., 1974. Microbiology and biochemistry. Mitt. int. Ver. Limnol. 20: 198–228.
Pettersson, K. & M. Jansson, 1978. Determinations of phosphatase activity in lake water — a study of methods. Verh. int. Verh. Limnol. 20: 1226–1230.
Psenner, R., R. Pucsko & M. Sager, 1984. Die Fraktionierung organischer und anorganischer Phosphorverbindungen von Sedimenten. Versuch einer Definition ökologisch wichtiger Faktoren. Arch. Hydrobiol./ Suppl. 70: 111–155.
Wetzel, R. G., 1990. Land-water interfaces: Metabolic and limnological regulators. Verh. int. Ver. Limnol. 24: 6–24.
Wetzel, R. G., 1991. Extracellular enzymatic interactions: Storage, redistribution, and interspecific communication. In R. J. Chrost (ed.), Microbial Enzymes in Aquatic Environments. Brock/Springer Series in Contemporary Bioscience. Springer-Verlag, New York: 6–28.
Williams, P. J. leB., 1973. The validity of the application of simple kinetic analysis to heterogeneous microbial populations. Limnol. Oceanogr. 18: 159–165.
Wright, R. T., 1973. Some difficulties in using 14C-organic solutes to measure heterotrophic bacterial activity. In L. H. Stevenson & R. R. Colwell (eds), Estuarine Microbial Ecology. University of South Carolina Press, Columbia, SC: 199–217.
Wright, R. T., 1974. Mineralization of organic solutes by heterotrophic bacteria. In R. R. Colwell & R. Y. Morita (eds), Effect of the Ocean Environment on Microbial Activities. University Park Press, Baltimore: 546–565.
Wright, R. T. & B. K. Burnison, 1979. Heterotrophic activity measured with radiolabelled organic substrates. In J. W. Costerton & R. R. Colwell (eds), Native Aquatic Bacteria: Enumeration, Activity, and Ecology. ASTM Special Technical Publication 695. American Society for Testing and MaterialsPhiladelphia: 140–155.
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Marxsen, J., Schmidt, HH. Extracellular phosphatase activity in sediments of the Breitenbach, a Central European mountain stream. Hydrobiologia 253, 207–216 (1993). https://doi.org/10.1007/BF00050739
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DOI: https://doi.org/10.1007/BF00050739