Abstract
The influence of a new micrografting method in vitro was tested by using long shoot meristems of adult 140 year old European larch trees grafted onto larch seedling rootstocks in sterile peat pellets. Five months after grafting and transfer of grafts to ex vitro conditions, new shoots from sprouting scions were re-established into tissue culture. The propagation behavior of shoots derived from these explants was compared with shoots established via bud culture from the same donor trees. Shoot explants from micrografts multiplied more rapidly than shoots explants from the original adult donor trees. Rooting experiments with cuttings from six micrografted clones resulted in 49.9 ± 11.9% rooting. Cuttings from donor trees invariably showed no root formation. The results confirm a rejuvenating influence of micrografting in larch.
Similar content being viewed by others
References
von Arnold S & Eriksson T (1981) In vitro studies of adventitious shoot formation in Pinus contorta. Can. J. Bot. 59: 870–874
Bonga JM & von Aderkas P (1993) Rejuvenation of tissues from mature conifers and its implications for propagation in vitro. In: Ahuja MR & Libby WJ (eds) Clonal Forestry, Vol 1 (pp 182–200). Springer-Verlag, Berlin Heidelberg New York
Boulay M (1979) Propagation ‘in vitro’ du Douglas par micropropagation de germination aseptique et culture de bourgeon dormants (Pseudotsuga menziesii (MIRB) Franco). In: Micropropagation d'arbres forestiers (pp 67–75). AFOCEL
Hübl S & Zoglauer K (1991) Entwicklung einer In-vitro-Vermehrungsmethode für züchterisch wertvolle Lärchen. Beitr. Forstwirtschaft 25 (1): 18–20
Kretzschmar U (1993) Improvement of larch micropropagation by induced short shoot elongation in vitro. Silvae Genet. 42 (4–5): 163–169
Kreizschmar U & Ewald D (1994) Vegetative propagation of 140-year-old Larix decidua trees by different in-vitro-techniques. J. Plant Physiol. 144 (4–5) 627–630
Monteuuis O (1987) Microgreffage de sequoia g'eant (pp 39–61). Ann. Rech. Sylvicoles 1986 AFOCEL
Monteuuis O (1991) Rejuvenation of a 100-year-old Sequoiadendron giganteum through in vitro meristem culture. I. Organogenic and morphological arguments. Physiol. Plant. 81: 111–115
Pliego-Alfaro F & Murashige T (1987) Possible rejuvenation of adult avocado by graftage onto juvenile rootstocks in vitro. Hort. Science 22 (6): 1321–1324
Trippi VS (1990) Ageing of meristems and morphogenetic potentialities. In: Rodriquez R; Tames RS & Durzan DJ (eds) Plant ageing. Basic and applied Approaches (pp 3–10). Plenum Press, New York and London
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ewaid, D., Kretzschmar, U. The influence of micrografting in vitro on tissue culture behavior and vegetative propagation of old European larch trees. Plant Cell Tiss Organ Cult 44, 249–252 (1996). https://doi.org/10.1007/BF00048531
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00048531