Skip to main content
SpringerLink
Log in

Thermoluminescence properties of the isolated photosystem two reaction centre

  • Chloroplasts
  • Regular Paper
  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

Formation of thermoluminescence signals is characteristics of energy- and charge storage in Photosystem II. In isolated D1/D2/cytochrome b-559 Photosystem II reaction centre preparation four thermoluminescence components were found. These appear at -180 (Z band), between -80 and -50 (Zv band), at -30 and at +35°C. The Z band arises from pigment molecules but not correlated with photosynthetic activity. The Zv and -30°C bands arise from the recombination of charge pairs stabilized in the Photosystem II reaction centre complex. The +35°C band probably corresponds to the artefact glow peak resulting from a pigment-protein-detergent interaction in subchloroplast preparations (Rózsa Zs, Droppa M and Horváth G (1989) Biochim Biophys Acta 973, 350–353).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Chl:

chlorophyll

Cyt:

cytochrome

DCMU:

3-(3,4-dichlorophenyl)-1,1′-dimethylurea

D1:

psbA gene product

D2:

psbD gene product

P680:

primary electron donor of PS II

Pheo:

pheophytin

PS II:

Photosystem II

QA :

primary quinone acceptor of PS II

QB :

secondary quinone acceptor of PS II

RC:

reaction centre of PS II

TL:

thermoluminescence

References

  • Barber J (1987) Photosynthetic reaction centres: a common link. Trends Biochem Sci 12: 321–326

    Google Scholar 

  • Barber J, Chapman DJ and Telfer A (1987a) Characterization of a PS II reaction centre isolated from the chloroplasts of Pisum sativum. FEBS Lett 220: 67–73

    Google Scholar 

  • Barber J, Gounaris K and Chapman DJ (1987b) Isolation of the Photosystem II reaction centre and the location and function of cytochrome b-559. In: Papa S et al. (eds) Cytochrome Systems: Molecular Biology and Bioenergetics, pp 657–666. New York: Plenum

    Google Scholar 

  • Chapman DJ, Gounaris K and Barber J (1988) Electron transport properties of the isolated D1-D2 cytochrome b-559 Photosystem II reaction centre. Biochim Biophys Acta 933: 423–431

    Google Scholar 

  • Danielius RV, Satoh K, vanKan PM, Plijter JJ, Nuijs AM and vanGorkom HJ (1987) The primary reaction of Photosystem II in the D1-D2-cytochrome b-559 complex. FEBS Lett 213: 241–244

    Google Scholar 

  • Debus RJ, Barry BA, Sithole I, Babcock GT and McIntosh L (1988a) Directed mutagenesis indicates that the donor to P680 + in Photosystem II is tyrosine-161 of the D1 polypeptide. Biochemistry 27: 9071–9074

    Google Scholar 

  • Debus RJ, Barry BA, Babcock GT and McIntosh L (1988b) Site-directed mutagenesis identifies a tyrosine radical involved in the photosynthetic oxygen-evolving system. Proc Natl Acad Sci USA 85: 427–430

    Google Scholar 

  • Demeter S, Rózsa Zs, Vass I and Sallai A (1985) Thermoluminescence study of charge recombination in Photosystem II at low temperatures. I. Characterization of the Zv and A thermoluminescence bands. Biochim Biophys Acta 809: 369–378

    Google Scholar 

  • Demeter S and Govindjee (1989) Thermoluminescence in plants. Physiol Plant 75, 121–130

    Google Scholar 

  • Gounaris K, Chapman DJ and Barber J (1988) Reconstitution of plastoquinone in the D1/D2/cytochrome b-559 Photosystem II reaction centre complex. FEBS Lett 240: 143–147

    Google Scholar 

  • Havemann J and Mathis P (1976) Flash-induced absorption changes of the primary donor of Photosystem II at 820 nm in chloroplasts inhibited by low pH or Tris-treatment. Biochim Biophys Acta 440: 346–355

    Google Scholar 

  • Inoue Y and Shibata K (1982) Thermoluminescence from photosynthetic apparatus. In: Govindjee (ed) Photosynthesis, Vol 1, pp 507–533. New York: Academic Press

    Google Scholar 

  • Kleinfeld D, Okamura MY and Feher G (1984) Electron-transfer kinetics in photosynthetic reaction centers cooled to cryogenic temperatures in the charge-separated state: Evidence for light-induced structural changes. Biochemistry 23: 5780–5786

    Google Scholar 

  • Klimov VV, allakhverdiev SI, Shafiev MA and Demeter S (1985) Effect of complete extraction and readdition of manganese on thermoluminescence of pea Photosystem II preparations. Biochim Biophys Acta 809: 414–420

    Google Scholar 

  • Koike H, Siderer Y, Ono T and Inoue Y (1986) Assignment of thermoluminescence A band to S3QA - charge recombination: sequential stabilization of S3 and QA by a two step illumination at different temperatures. Biochim Biophys Acta 850: 80–89

    Google Scholar 

  • Michel H and Deisenhofer J (1986) X-ray diffraction studies on a crystalline bacterial photosynthetic reaction center: A progress report and conclusions on the structure of Photosystem II reaction center. In: Staehelin AL and Arntzen CJ (eds) Encyclopedia Plant Physiol Photosynthesis III, Vol 19, pp 371–381. Berlin: Springer

    Google Scholar 

  • Michel H and Deisenhofer J (1988) Relevance of the photosynthetic reaction center from purple bacteria to the structure of Photosystem II. Biochemistry 27: 1–7

    Google Scholar 

  • Nanba O and Satoh K (1987) Isolation of a Photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559. Proc Natl Acad Sci USA 84: 109–112

    Google Scholar 

  • Newell WR, vanAmerongen H, vanGrondelle R, Aalberts JW, Drake AF, Udvarhelyi P and Barber J (1988) Spectroscopic characterization of the reaction centre of Photosystem II from higher plants. FEBS Lett 228: 162–166

    Google Scholar 

  • Okamura MY, Satoh K, Isaacson RA and Feher G (1987) Evidence of the primary charge separation in the D1D2 complex of Photosystem II from spinach: EPR of the triplet state. In: Biggins J (ed) Progress in Photosynthesis Research Vol 1, pp 379–381. Dordrecht: Martinus Nijhoff

    Google Scholar 

  • Rózsa Zs, Droppa M and Horváth G (1989) On the origin of the thermoluminescence band at around +50°C in isolated subchloroplast particles. Biochim Biophys Acta 973: 350–353

    Google Scholar 

  • Sane PV and Rutherford AW (1986) Thermoluminescence from photosynthetic membranes. In: Govindjee, Amesz J and Fork DC (eds) Light Emission by Plants and Bacteria, pp 329–360. New York: Academic Press

    Google Scholar 

  • Takahashi Y, Hansson O, Mathis P and Satoh K (1987) Primary radical pair in the Photosystem II reaction centre. Biochim Biophys Acta 893: 49–59

    Google Scholar 

  • Telfer A, Barber J and Evans MCV (1988) Oxidation-reduction potential dependence of reaction centre triplet formation in the isolated D1/D2/cytochrome b-559 Photosystem II complex. FEBS Lett 232: 209–213

    Google Scholar 

  • Trebst A (1986) The topology of the plastoquinone and herbicide binding peptides of Photosystem II in the thylakoid membrane. Z Naturforsch 41c: 240–245

    Google Scholar 

  • vanDorssen RJ, Breton J, Plijter JJ, Satoh K, vanGorkom HJ and Amesz J (1987) Spectroscopic properties of the reaction center and of the 47 kDa chlorophyll protein of Photosystem II. Biochim Biophys Acta 893: 267–274

    Google Scholar 

  • Vass I, Horváth G, Herczeg T and Demeter S (1981) Photosynthetic energy conservation investigated by thermoluminescence. Activation energies and half-lives of thermoluminescence bands of chloroplasts determined by mathematical resolution of glow curves. Biochim Biophys Acta 634: 140–152

    Google Scholar 

  • Vass I, Rózsa Zs and Demeter S (1984) Flash-induced oscillation of the low temperature thermoluminescence band Zv in chloroplasts. Photochem Photobiol 40: 407–409

    Google Scholar 

  • Vermaas WFJ, Rutherford AW and Hansson Ö (1988) Site-directed mutagenesis in Photosystem II of the cyanobacterium Synechocystis sp PCC 6803: Donor D is a tyrosine residue in the D2 protein. Proc Natl Acad Sci USA 85: 8477–8481

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary

    Imre Vass

  2. AFCR Photosynthesis Research Group, Department of Pure and Applied Biology, Imperial College, SW7 2BB, London, U.K.

    David J. Chapman & James Barber

Authors
  1. Imre Vass
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. David J. Chapman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. James Barber
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vass, I., Chapman, D.J. & Barber, J. Thermoluminescence properties of the isolated photosystem two reaction centre. Photosynth Res 22, 295–301 (1989). https://doi.org/10.1007/BF00048307

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00048307

Key words

Search

Navigation