Abstract
Cultured caryopses of finger millet (Eleusine coracana GAERTN) produced callus from shoot apices or mesocotyls depending upon the concentration of picloram and combination of cytokinins in MS basal medium. On subsequent subcultures, numerous somatic embryos differentiated from the callus on MS medium supplemented with picloram and kinetin. The embryos germinated into complete plants on medium devoid of phytohormones. When different carbohydrates were tested, basal medium containing glucose and sucrose produced the highest frequency of germinating somatic embryos. Supplementation of MS basal medium with a variety of aminoacids, osmotic agents and growth supplements had an adverse effect on the germination of embryos. Incorporation of different antibiotics such as carbenicillin, cefotaxime and streptomycin sulfate enhanced plant differentiation from somatic embryos. Cytological analysis of regenerated plants showed normal diploid chromosome number in their root tips.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxy acetic acid
- BA:
-
benzyl adenine
- 2,iP:
-
6-γ-γ-dimethylallylamino purine
- Kn:
-
kinetin
- Z:
-
zeatin
References
Armstrong, CL & Green, CE (1985) Establishment and maintenance of friable embryogenic maize callus and the involvement of L-proline. Planta 164: 207–214
Collins, GB, Vian, WE & Phillips, GC (1978) Use of 4-amino-3,5,6-trichloropicolinic acid as an auxin source in plant tissue cultures. Crop Sci. 18: 286–288
Eapen, S & George, L (1989) High frequency plant regeneration through somatic embryogenesis in finger millet (Eleusine coracana GAERTN). Plant Sci. 61: 127–130
Gleddie, S, Keller, W & Setterfield, G (1983) Somatic embryogenesis from leaf explants and cell suspensions of Solanum melongena (eggplant). Can. J. Bot. 61: 656–666
Green CE (1978) In vitro plant regeneration in cereals and grasses. In: Thorpe TA (Ed) Frontiers of Plant Tissue Culture (pp411–418). Univ. of Calgary
Hanna, W, Lu, C & Vasil, IK (1984) Uniformity of plants regenerated from somatic embryos of Panicum maximum Jacq (Guinea grass). Theor. Appl. Genet. 61: 155–159
Kavi Kishor, PB (1987) Energy and osmotic requirement for high frequency regeneration of rice plants from long term cultures. Plant Sci. 48: 189–194
Luhrs, R & Lorz, H (1987) Plant regeneration in vitro from embryogenic cultures of spring and winter type barley (Hordeum vulgare L.) Theor. Appl. Genet. 75: 16–25
Mathias, RJ & Boyd, LA (1986) Cefotaxim on callus growth, embryogenesis and regeneration in hexaploid breadwheat (Triticum aestivum L. cm. Thell). Plant Sci. 46: 217–223
Mathias, RJ & Mukasa, C (1987) The effect of cefotaxim on the growth and regeneration of callus from four varieties of barley (Hordeum vulgare L.). Plant Cell Rep. 6: 457
Murashige, T & Skoog, F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Mohanty, BD, Dutta, Gupta S & Ghosh, PD (1985) Callus initiation and plant regeneration in ragi (Eleusine coracana Gaertn). Plant Cell, Tiss. Org. Cult. 5: 147–150
Nitsch C (1974) Pollen culture-a new technique for mass production of haploid and homozygous plants. In: Proc. of International Symp on haploids in higher plants (pp 123–125). University of Guelph
Owens, LD (1979) Kanamycin promoted morphogenesis of plant tissues. Plant Sci. Lett. 16: 225–230
Rangan, TS (1976) Growth and plantlet regeneration in tissue cultures in some Indian Millets Paspalum scrobiculatum L., Eleusine coracana Gaertn and Pennisetum typhoideum Pers. Z. Pflanzenphysiol. 78: 208–216.
Siriwardana, S & Nabros, MW (1983) Tryptophan enhancement of somatic embryogenesis in rice. Plant Physiol. 73: 142–146.
Sozinov, A, Lukyanyuk, S & Ignatova, S (1981) Anther cultivation and induction of haploid plant in triticale. Z. Pflanzenzuchtg. 86: 272–285
Strickland, SG, Nichol, JW, McCall, CM & Stuart, DA (1987) Effect of carbohyrate source on alfalfa somatic embryogenesis. Plant Sci. 48: 113–121
Swedlund, B & Vasil, IK (1985) Cytogenetic characterisations of embryogenic callus and regenerated plants of Pennisetum americanum (L) K. Schum. Theor. Appl. Genet. 69: 575–581
Vasil IK (1985) Somatic embryogenesis and its consequences in Gramineae. In: Henke RR et al. (Eds) Tissue Culture in Forestry and Agriculture (pp 31–47). Plenum Publishing Corp.
Valcerde, R, Arias, O & Thorpe, TA (1987) Picloram induced somatic embryogenesis in pejibaye palm (Bactris gasipaes HBK). Plant Cell, Tiss. Org. Cult. 10: 149–156
Wakisuka, T & Yamaguchi, T (1987) The induction of enlarged apical domes in vitro and multi-shoot formations from finger millet (Eleusine coracana). Ann. Bot. 60: 331–336
Xu, ZH & Sunderland, N (1981) Glutamine, inositol and conditioning factor in the production of barley pollen callus in vitro. Plant Sci. Lett. 23: 161–168
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Eapen, S., George, L. Influence of phytohormones, carbohydrates, aminoacids, growth supplements and antibiotics on somatic embryogenesis and plant differentiation in finger millet. Plant Cell Tiss Organ Cult 22, 87–93 (1990). https://doi.org/10.1007/BF00043683
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DOI: https://doi.org/10.1007/BF00043683