Abstract
Anthers of diploid genotypes of Solanum tuberosum capable of androgenesis were cultured on different media to examine the effect on induction of pollen embryogenesis of 2,4-d and lactose. Anthers cultured in callogenic medium with 2,4-d and sucrose produced pollen derived embryoids only exceptionally. When sucrose was replaced by lactose the frequency of embryogenesis was as high or higher than in embryogenic auxin-free medium. Substitution of lactose for sucrose in the embryogenic medium had no effect. Supplementing the embryogenic medium with 2,4-d strongly reduced the frequency of pollen embryoids in the presence of sucrose but not with lactose.
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Abbreviations
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
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Říhová, L., Tupý, J. Influence of 2,4-d and lactose on pollen embryogenesis in anther culture of potato. Plant Cell Tiss Organ Cult 45, 269–272 (1996). https://doi.org/10.1007/BF00043641
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DOI: https://doi.org/10.1007/BF00043641