Abstract
Efficient in vitro plant regeneration systems are critical for many purposes including plant transformation. Current regeneration systems for melon (Cucumis melo L.) plants generally utilize cotyledon explants; regeneration from melon leaves has received limited attention. We investigated several factors that influence regeneration from melon leaves including: genotype growth conditions and age of the source plant, leaf age, explant orientation, gelling agent, and the addition of silver nitrate and sulfonylurea herbicide to the culture media. Critical factors that influenced regeneration were preculture conditions of the donor plants, leaf size, and the use of silver nitrate and Phytagel in the medium. The best results were obtained with 3–4 cm diam leaves excised from pot grown greenhouse or growth chamber plants cultured on MS medium with 5 μM IAA, 5 μM BA, 1 μM ABA, 30 μM silver nitrate and 2.6 g l-1 Phytagel. Low concentratons of sulfonylurea herbicide (0.25 mg l-1 DPX-M 6316) also enhanced regeneration. Under optimized conditions 80–100% of the explants regenerated, with 10–100 shoots per explant
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Abbreviations
- ABA:
-
abscisic acid
- BA:
-
benzyladenine
- IAA:
-
indole-3-acetic acid
- MS:
-
Murashige and Skoog medium
- NAA:
-
naphthalene acetic acid
References
Beyer EM (1976) Silver ion: a potent anti-ethylene agent in cucumber and tomato. HortScience 11: 195–196
Brown DC & Thorpe TA (1986) Plant regeneration by organogenesis. In: Vasil IK (ed) Somatic cell genetics of plants, Vol 3 (pp 49–65). Academic Press Inc, New York
Bornman CH & Vogelmann TC (1984) Effect of rigidity of gel medium on benzyladenine-induced adventitious bud formation and vitrification in vitro in Picea abies. Physiol Plant. 61: 502–512
Ciardi T, Carputo D & Frusciante L (1992) In vitro shoot regeneration and chromosome doubling in 2X and 3X potato clones. Potato J. 69: 1–12
Chaleff R & Mauvais CJ (1984) Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science 224: 1443–1445
Chee PP (1991) Plant regeneration from cotyledons of Cucumis melo ‘Topmark’. HortScience 26: 908–910
Chraibi KMB, Castelle JC, Latche A, Roustan JP & Fallot J (1992) A genotye independent system of regeneration from cotyledons of sunflower (Helianthus annus L.). The role of ethylene. Plant Science 86: 215–221
Debeaujon I & Branchard M (1992) Induction of somatic embryogenesis and caulogenesis from cotyledon and leaf protoplast-derived colonies of melon (Cucumis melo L.). Plant Cell Rep. 12: 37–40
Dirks R & van Buggenum M (1989) In vitro plant regeneration from leaf and cotyledon explants of Cucumis melo L. Plant Cell Rep. 7: 626–627
Dong JZ, Yang MZ, Jia SR & Chua NH (1991) Transformation of melon (Cucumis melo L.) and expression from the cauliflower mosaic virus 35S promoter in transgenic melon plants. Bio/Technology 9: 858–863
Ezura HH, Amagai D, Yoshioka D & Oosawa K (1992) Highly frequent appearance of tetraploidy in regenerated plants, a universal phenomenon in tissue cultures of melon (Cucumis melo). Plant Sci. 85: 209–213
Fang G & Grumet R (1990) Agrobacterium tumefaciens mediated transformtion and regeneration of muskmelon plants. Plant Cell Rep. 9: 160–164
Fang G & Grumet R (1993) Genetic engineering of potyvirus resistance using constructs derived from the zucchini yellow mosaic virus coat protein gene. Molec. Plant Microbe Interact. 6: 358–367
Fassuliotus G & Nelson BV (1992) Regeneration of tetraploid muskmelons from cotyledons and their morphological differences from two diploid muskmelon genotypes. J. Amer. Soc. Hort. Sci. 117: 863–866
Gleddie S (1989) Plant regeneration from cell suspension cultures of Arabidopsis thaliana heynh. Plant Cell Rep. 8: 1–5
Gray DJ, McColley DW & Compton ME (1993) High-frequency somatic embryogenesis from quiescent seed cotyledons of Cucumis melo cultivars. J. Amer. Soc. Hort. Sci. 118: 425–432
Gonsalves C, Zue B, Yepes M, Fuchs M, Ling K, Namba S, Chee P, Slightom JL & Gonsalves D (1994) Transferring cucumber mosaic virus-white leaf strain coat protein gene into Cucumis melo L. and evaluating transgenic plants for protection against infections. J. Amer. Soc. Hort. Sci. 119: 345–355
Hammar S & Grumet R (1990) Regeneration and Agrobacterium tumefaciens-mediated transformation of cucumber (Cucumis sativus L.). Hortscience 25: 1070
Kathal R, Bhatnagar SP & Bhojwani SS (1986) Regeneration of shoots from hypocotyl callus of Cucumis melo cv. Pusa Sharbarti. J. Plant Physiol. 126: 59–62
Kathal R, Bhatnagar SP & Bhojwani SS (1988) Regeneration of plants from leaf explants of Cucumis melo cv. Pusa Sharbati. Plant Cell Rep. 7: 449–451
Kathal R, Bhatnagar SP & Bhojwani SS (1992) Chromosome variations in the plants regenerated from leaf explants of Cucumis melo L. cv. ‘Pusa Sharbati’. Caryologia 45: 51–56
Kathal R, Bhatnagar SP & Bhojwani SS (1994) Plant regeneration from the callus derived from root explants of Cucumis melo cv. Pusa Sharbati. Plant Sci. 96: 137–142
Li R, Sun Y, Zhang L & Li X (1990) Plant regeneration from cotyledon protoplasts of Xinjiang muskmelon. Plant Cell Rep. 9: 199–203
Miki BL, Labbe H, Hatton J, Ouellet T, Gabard J, Sunohara G, Charest PJ & Iyer VN (1990) Transformation of Brassica napus canola cultivars with Arabidopsis thaliana acetohydroxy acid synthase genes and analysis of herbicide resistance. Theor. Appl: Genet. 80: 449–459
Moreno V, Garcia-Sogo M, Grannell I, Garcia-Sogo B & Roig LA (1985) Plant regeneration from calli of melon (Cucumis melo L. cv. Amarillo Oro). Plant Cell Tiss. Org. Cult. 5: 139–146
Murashige T & Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue cultures. Physiol. Plant. 15: 473–498
Neidz RP, Smith SS, Dunbar KB, Stephens CT & Murakishi HH (1989) Factors influencing shoot regeneration from cotyledonary explants of Cucumis melo. Plant Cell. Tiss. Org. Cult. 18: 313–319
Oridate T & Oosawa K (1986) Somatic embryogenesis and plant regeneration from suspension callus culture in melon (Cucumis melo L.). Jpn. J. Breed. 36: 424–428
Roustan JP, Latche A & Fallot J (1992) Enhancement of shoot regeneration from cotyledons of Cucumis melo by AgNO3, an inhibitor of ethylene action. J. Plant Physiol. 140: 485–488
Sharma KK, Bhojwani SS & Thorpe TA (1990) Factors affecting high frequency differentiation of shoots and roots from cotyledon explants of Brassica juncea L. Czern. Plant Sci. 66: 247–253
Steele RGD & Torrie JH (1960) Principles and Procedures of Statistics. McGraw-Hill Book Co., New York
Tabei Y, Kanno T & Nishio T (1991) Regulation of organogenesis and somatic embryogenesis by auxin in melon, Cucumis melo. L. Plant Cell Rep. 10: 225–229
Valles MP & Lasa JM (1994) Agrobacterium-mediated transformation of commercial melon (Cucumis melo L. cv. Amarillo Oro). Plant Cell Rep. 13: 145–148
Yadav RC & Grumet R (1994) Tendrils as an alternate tissue source for chromosome visualization. J. Amer. Soc. Hort. Sci. 119: 850–852
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Yadav, R.C., Saleh, M.T. & Grumet, R. High frequency shoot regeneration from leaf explants of muskmelon. Plant Cell Tiss Organ Cult 45, 207–214 (1996). https://doi.org/10.1007/BF00043632
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DOI: https://doi.org/10.1007/BF00043632