Abstract
Callus cultures were initiated from leaf sections of raspberry (Rubus idaeus L.) cv. Royalty. Explants from younger leaves produced significantly more calli than those from older leaves. Anderson's salt mixture was more efficient for callus induction than the Murashige-Skoog medium. The best propagation and growth of calli was observed on Anderson's medium supplemented with 9 μM 2,4-dichlorophenoxyacetic acid, 4.9 μM indolebutyric acid and 4.9 μM 6-(dimethylallylamino)-purine. During a 28-day period, the fresh weight of calli increased approximately five times. The same medium without agar was used for establishing cell suspension cultures. Fresh weight of cells increased four times and dry weight approximately doubled during 10 days of culture.
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Abbreviations
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- 2iP:
-
6-(dimethylallylamino)-purine
- IBA:
-
indolebutyric acid
- MS:
-
Murashige & Skoog basal salt medium
References
Anderson, WC (1980) Tissue culture propagation of red and black raspberries, Rubus idaeus and R. occidentalis. Acta Hort. 112: 13–20
Borgman, CA & Mudge, KW (1986) Factors affecting the establishment and maintenance of Titan red raspberry root organ cultures. Plant Cell Tiss. Org. Cult. 6: 127–137
Hahlbrock, K (1975) Further studies on the relationship between the rates of nitrate uptake growth and conductivity changes in the medium of plant cell suspension culture. Planta 124: 311–318
Murashige, T & Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Singha, S, Oberly, GH & Townsend, EC (1987) Changes in nutrient composition and pH of the culture medium during in vitro shoot proliferation of crabapple and pear. Plant Cell Tiss. Org. Cult. 11: 209–220
Stepan-Sarkissian, G & Grey, D (1990) Growth determination and medium analysis. In: Pollard, JW & Walker, JM (Eds) Methods in Molecular Biology, Plant Cell and Tissue Culture, Vol. 6 (pp 13–27) The Humana Press, Clifton, New Jersey
Taylor, PWJ, Ko, HL, Adkins, SW, Rathus, C & Birch, RG (1992) Establishment of embryogenic callus and high protoplast yielding suspension cultures of sugarcane (Saccharum spp. hybrids). Plant Cell Tiss. Org. Cult. 28: 69–78
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Berejsza-Wysecki, W., Hrazdin, G. Establishment of callus and cell suspension cultures of raspberry (Rubus idaeus cv. Royalty). Plant Cell Tiss Organ Cult 37, 213–216 (1994). https://doi.org/10.1007/BF00043620
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DOI: https://doi.org/10.1007/BF00043620