Abstract
A protocol is described for the production of plantlets from mature excised embryos of jack pine (Pinus banksiana Lamb.), a conifer widely distributed in temperate North America. Shoot buds were induced on von Arnold and Erickson's or Bornman's MCM salts with 10 μM cytokinin for 2 weeks, using Phytagar® for gelling the medium. Bud development and shoot elongation required frequent subculture on MCM medium with activated charcoal and reduced inorganic nitrogen during elongation. Shoots were rooted in peat-perlite with α-naphthaleneacetic acid. The protocol produces about six plantlets per embryo.
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Harry, I.S., Thorpe, T.A. Regeneration of plantlets through organogenesis from mature embryos of jack pine. Plant Cell Tiss Organ Cult 37, 159–164 (1994). https://doi.org/10.1007/BF00043610
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DOI: https://doi.org/10.1007/BF00043610