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Plant regeneration from stem and petiole protoplasts of sweet potato (Ipomoea batatas) and its wild relative, I. lacunosa

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Abstract

A protoplast-to-plant regeneration system has been established for sweet potato (Ipomoea batatas (L.) Lam.) and its wild relative, I. lacunosa L. Viable protoplasts, isolated from preplasmolyzed stems and petioles of in vitro-grown plants, were cultured on liquid MS (Murashige & Skoog 1962) medium that supported cell division and colony formation. Embryogenic calli of sweet potato were induced on agar-solidified MS medium supplemented with 3% (w/v) sucrose, 50 mg l-1 casamino acids, 0.2–0.5 mg l-1 2,4-d, 1.0 mg l-1 kinetin and 1.0 mg l-1 ABA. On average, 3 plants were regenerated from a single sweet potato callus subcultured on semi-solid MS medium containing 3% (w/v) sucrose, 800 mg l-1 glutamine, 2.0 mg l-1 BA or 1.0 mg l-1 kinetin and 1.0 mg l-1 GA3. Embryogenic calli of I. lacunosa L. were initiated on semi-solid MS medium containing 0.2–0.5 mg l-1 IAA and 1.0–2.0 mg l-1 BA. An average of 5 plants was regenerated from a single sweet potato callus subcultured on semi-solid MS medium containing 0.5 or 1.0 mg l-1 GA3.

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Abbreviations

ABA:

abscisic acid

BA:

benzyladenine

2,4-d :

2,4-dichlorophenoxyacetic acid

GA3 :

gibberellic acid

IAA:

indole acetic acid

MES:

2-(N-morpholino)-ethane sulfonic acid

NAA:

α-naphthaleneacetic acid

References

  • Belarmino, MM, Abe, T & Sasahara, T (1992) Efficient plant regeneration from leaf calli of Ipomoea batatas (L.) Lam and its related species. Japan. J. Breed. 42: 109–114

    Google Scholar 

  • Chee, RP & Cantliffe, DJ (1988) Somatic embryony patterns and plant regeneration in Ipomoea batatas Poir. In Vitro Cell. Dev. Biol. 24: 955–958

    Google Scholar 

  • Chee, RP, Leskovar, DI & Cantliffe, DJ (1992) Optimizing embryogenic callus and embryo growth of a synthetic seed system for sweet potato by varying media nutrient concentrations. J. Amer. Soc. Hort. Sci. 117: 663–667

    Google Scholar 

  • Cocking, EC (1972) Plant cell protoplasts-isolation and development. Ann. Rev. Plant Physiol. 23: 29–50

    Google Scholar 

  • Etienne, H, Sota, B, Montoro, P, Miginiac, E & Carron, MP (1993) Relations between exogenous growth regulators and endogenous indole-3-acetic acid and abscissic acid in the expression of somatic embryogenesis in Hevea brasiliensis (Mull. Arg.). Plant Sci. 88: 91–96

    Google Scholar 

  • Faiz, ZO & Torello, WA (1992) Plant regeneration from callus and protoplasts of perennial ryegrass (Lolium perenne L.). J. Plant Physiol. 140: 101–105

    Google Scholar 

  • Gupta, V, Agnihotri, A & Jagannathan, V (1990) Plant regeneration from callus and protoplasts of Brassica nigra (IC 257) through somatic embryogenesis. Plant Cell Rep. 9: 427–430

    Google Scholar 

  • Iwanaga M (1987) Use of wild germplasm for sweet potato breeding. Rep. 1st Sweet Potato Planning Conf., CIP, Lima, Peru. 27–59

  • Kirti, PB & Chopra, VL (1990) Rapid plant regeneration through organogenesis and somatic embryo- genesis from cultured protoplasts. Plant Cell Tiss. Org. Cult. 20: 65–67

    Google Scholar 

  • Liu, QC, Kokubo, T & Sato, M (1991) Plant regeneration from Ipomoea triloba L. protoplasts. Japan. J. Breed. 41: 103–108

    Google Scholar 

  • Liu, QC (1992) Shoot regeneration from protoplast fusions of sweet potato and its related species. Japan J. Breed. 42 (suppl. 1): 88–82

    Google Scholar 

  • Lu Sy, Shen GL & Song BF (1987) The goals of sweet potato breeding in China. Rep. 1st Sweet Potato planning Conf., CIP, Lima, Peru. 247–260

  • Murashige, T & Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Murata, T, Hoshino, K & Miyaji, Y (1987) Callus formation and plant regeneration from petiole protoplast of sweet potato, Ipomoea batatas (L.) Lam Japan J. Breed. 37: 291–298

    Google Scholar 

  • Murata, T, Fukuoka, H & Kishimoto, M (1991) Medium condition on plant regeneration from protoplast of sweet potato. Japan J. Breed. 41 (suppl.1): 54–55

    Google Scholar 

  • Murata, T (1993) Plant regeneration from fused cells of sweet potato. Japan J. Breed. 43 (suppl.1): 20–21

    Google Scholar 

  • Nishiyama, I, Miyazaki, T & Sakamoto, S (1975) Evolutionary autoploidy in the sweet potato (Ipomoea batatas (L.) Lam.) and its progenitors. Euphytica 24: 197–208

    Google Scholar 

  • Ochatt, SJ, Chevreau, E & Gal let, M (1992) Organogenesis from ‘Passe Crassane’ and ‘Old Home’ pear (Pyrus communis L.) protoplasts and isoenzymatic trueness-to-type of the regenerated plants. Theor. Appl. Genet. 83: 1013–1018

    Google Scholar 

  • Orjeda, G, Freye, R & Iwanaga, M (1991) Use of Ipomoea trifida germ plasm for sweet potato improvement. 3. Development of 4x interspecific hybrids between Ipomoe batatas (L.) Lam. (2n=6x=90) and I. trifida (H.B.K) G. Don. (2x=2x=30) as storage-root initiators for wild species. Theor. Appl. Genet. 83: 159–163

    Google Scholar 

  • Pattat-Ochatt, EM, Ochatt, SJ & Power, JB (1988) Plant regeneration from protoplasts of apple root-stocks and scion varieties (Malus x domestica Borkh). J. Plant Physiol. 133: 460–465

    Google Scholar 

  • Reyes, LM & Collins, W (1992) Genetic control of seven enzyme systems in Ipomoea species. J. Amer. Horti. Sci. 117: 1000–1005

    Google Scholar 

  • Sakamoto, S (1976) Breeding of a new sweet potato variety, Minamiyutaka, by the use of wild relatives. Japan. Agr. Res. Quart. 10: 183–186

    Google Scholar 

  • Shepard, J F (1980) Abscisic acid-enhanced shoot initiation in protoplast derived calli of potato. Plant Sci. Lett. 18: 327–333

    Google Scholar 

  • Shepard, JF (1981) Protoplasts as source of disease resistance in plants. Ann. Rev. Plant Physiol. 19: 145–166

    Google Scholar 

  • Shepard, JF & Totten, RE (1975) Mesophyll cell protoplasts of potato: Isolation, proliferation, and plant regeneration. Plant Physiol. 60: 313–316

    Google Scholar 

  • Shimonishi, K, Karube, M & Kukimura, H (1992) Rapid embryogenesis by NAA and plant regeneration in sweet potato. Japan. J. Breed. 42 (suppl. 1): 60–61

    Google Scholar 

  • Shiotani, I, Yoshida, S & Kawase, T (1990) Numerical taxonomic analysis and crossability of diploid Ipomoea species related to the sweet potato. Japan. J. Breed. 40: 159–174

    Google Scholar 

  • Sihachakr, D & Ducreux, G (1987) Plant regeneration from protoplast culture of sweet potato (Ipomoea batatas Lam.). Plant Cell Rep. 6: 326–328

    Google Scholar 

  • Suga, R, Takagi, Y & Okutsu, Y (1990) Protoplast culture of sweet potato and its related wild species. Japan J. Breed. 40 (suppl.1): 78–79

    Google Scholar 

  • Wallin, A & Johansson, L (1989) Plant regeneration from leaf mesophyll protoplasts of in vitro cultured shoots of a columnar apple. J. Plant Physiol. 135: 565–570

    Google Scholar 

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Author notes

  1. M. M. Belarmino

    Present address: Philippine Root Crop Research & Training Center, ViSCA, 6521-A, Leyte, Philippines

Authors and Affiliations

  1. Laboratory of Plant Breeding, Faculty of Agriculture, Yamagata University, 997, Tsuruoka, Japan

    M. M. Belarmino, T. Abe & T. Sasahara

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  1. M. M. Belarmino
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  2. T. Abe
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  3. T. Sasahara
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Belarmino, M.M., Abe, T. & Sasahara, T. Plant regeneration from stem and petiole protoplasts of sweet potato (Ipomoea batatas) and its wild relative, I. lacunosa . Plant Cell Tiss Organ Cult 37, 145–150 (1994). https://doi.org/10.1007/BF00043608

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  • DOI: https://doi.org/10.1007/BF00043608

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