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Somatic hybridization between Solanum ochranthum and Lycopersicon esculentum

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Abstract

Incorporation of genes from wild species has been a major contributor to tomato improvement in recent years. Solanum ochranthum, a woody vine-like tomato relative, is a potential source of resistance against tomato diseases and insect pests but is genetically isolated from tomato. Somatic hybridization methods were developed to facilitate the use of S. ochranthum for tomato germplasm improvement. Leaf mesophyll protoplasts of S. ochranthum and selected Lycopersicon esculentum genotypes were chemically fused with polyethylene glycol. The protoplasts were initially cultured in Shepard's CL, a Murashige and Skoog-based medium, containing 1 mg l-1 NAA, 0.5 mg l-1 N6-benzyladenine and 0.5 mg l-1 2,4-dichlorophenony-acetic acid. Tetraploid and hexaploid hybrid regenerants and regenerants of an L. esculentum parent were recovered; S. ochranthum did not regenerate. Hybridity was established by morphological characters, peroxidase isozyme and RAPD markers.

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Abbreviations

MS:

Murashige and Skoog (1962) medium

CL:

Shepard (1980) cell layer medium

2,4-d :

2,4-dichlorophenoxyacetic acid

NAA:

α-naphthaleneacetic acid

BAP:

N6-benzyladenine

MES:

2-N-morpholinoethanesulfonic acid

PEG:

polyethylene glycol

RAPD:

randomly amplified polymorphic DNA

PPM:

potato propagation medium

TPM:

tomato propagation medium

OM:

modified Murashige and Skoog (1962) medium

OM + AC:

modified Murashige & Skoog (1962) medium + activated charcoal

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Authors and Affiliations

  1. Agricultural Research Service, Plant Science Institute, Vegetable Laboratory, U.S. Department of Agriculture, 20705, Beltsville, MD, USA

    Ruth S. Kobayashi (Research Plant Geneticist) & John R. Stommel (Research Plant Geneticist)

  2. Agricultural Research Service, Plant Science Institute, Vegetable Laboratory, U.S. Department of Agriculture, 20705, Beltsville, MD, USA

    Stephen L. Sinden (Research Plant Physiologist)

Authors
  1. Ruth S. Kobayashi
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  2. John R. Stommel
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  3. Stephen L. Sinden
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Kobayashi, R.S., Stommel, J.R. & Sinden, S.L. Somatic hybridization between Solanum ochranthum and Lycopersicon esculentum . Plant Cell Tiss Organ Cult 45, 73–78 (1996). https://doi.org/10.1007/BF00043431

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  • DOI: https://doi.org/10.1007/BF00043431

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