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Callus cultures of Artemisia absinthium L.: initiation, growth optimization and organogenesis

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Abstract

Callus cultures were initiated from micropropagated Artemisia absinthium plantlets on MS basal medium supplemented with different concentrations of BA, Kn, NAA, IAA and 2,4-d in combination or singly. Supplementing the medium with low doses of both BA in combination with NAA, and Kn in combination with NAA enhanced the growth rate of callus cultures. However, cultures grew slowly following the second subculture and the majority turned brown and died within the next month. Initiation of root and shoot primordia occured directly from leaf explants cultured on 1.81 μM 2,4-d, while adventitious shoot formation from callus was observed occasionally when BA was added to the medium in combination with IAA. Furthermore, medium containing 2.22 μM BA and 2.69 μM NAA stimulated both callus growth and organogenesis on some callus cultures derived from leaves and stems of young stock material. The best results were obtained with leaf explants. Cytological analysis of root meristems revealed that all regenerants were diploid (2n=18), as expected.

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Abbreviations

MS:

Murashige & Skoog's salts and vitamins (1962)

BA:

6-benzyladenine

NAA:

alphanaphthaleneacetic acid

Kn:

Kinetin (6-furfurylaminopurine)

2,4-d :

2,4-dichlorophenoxyacetic acid

IAA:

indoleacetic acid

FW:

fresh weight

Bi:

biomass increase

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Authors and Affiliations

  1. Dipartimento di Ortoflorofrutticoltura, Università di Firenze, Via Donizetti 6, 50144, Firenze, Italy

    Stefania Nin

  2. Dipartimento di Biologia Vegetale, Università di Firenze, Piazzale delle Cascine 28, 50144, Firenze, Italy

    Elena Morosi, Silvia Schiff & Andrea Bennici

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  1. Stefania Nin
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  2. Elena Morosi
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  3. Silvia Schiff
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  4. Andrea Bennici
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Nin, S., Morosi, E., Schiff, S. et al. Callus cultures of Artemisia absinthium L.: initiation, growth optimization and organogenesis. Plant Cell Tiss Organ Cult 45, 67–72 (1996). https://doi.org/10.1007/BF00043430

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  • DOI: https://doi.org/10.1007/BF00043430

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