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Regeneration of albino plantlets from suspension culture derived protoplasts of Poa pratensis L. (Kentucky bluegrass)

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Summary

A procedure for the regeneration of callus and albino plantlets from protoplasts of Kentucky bluegrass (Poa pratensis L.), cultivar Kimono, is described. Protoplasts were isolated from a suspension culture with shoot-forming ability, which had been initiated from immature inflorescence derived callus. Protoplasts were cultured in modified Kao & Michayluk (KM8P) liquid medium. Plantlets were recovered from protoplast-derived callus growing on hormone-free modified Murashige & Skoog (MS) medium. While the plantlets derived from the original callus cultures were green, those from suspension cultures and suspension culture derived protoplasts were albino.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

AA:

Amino acid medium (Müller & Grafe, 1978)

MS:

Murashige & Skoog (1962) medium

KM8P:

Kao & Michayluk (1975) medium

MES:

2-(N-Morpholino)ethane sulfonic acid

N6 :

medium of Chu et al. (1975)

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Authors and Affiliations

  1. Foundation for Agricultural Plant Breeding, P. O. Box 117, 6700 AC, Wageningen, The Netherlands

    P. van der Valk & M. A. C. M. Zaal

  2. Barenbrug Holland BV, Oosterhout, The Netherlands

    J. Creemers-Molenaar

Authors
  1. P. van der Valk
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  2. M. A. C. M. Zaal
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  3. J. Creemers-Molenaar
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van der Valk, P., Zaal, M.A.C.M. & Creemers-Molenaar, J. Regeneration of albino plantlets from suspension culture derived protoplasts of Poa pratensis L. (Kentucky bluegrass). Euphytica 39 (Suppl 3), 169–176 (1988). https://doi.org/10.1007/BF00043380

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  • DOI: https://doi.org/10.1007/BF00043380

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