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Rapid multiplication of Sapium sebiferum Roxb. by tissue culture

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Abstract

Multiple shoots formation and elongation was induced from stem explants of Sapium seedlings on media containing cytokinins.

Leaf explants produced callus on a medium containing cytokinins, auxin, casein hydrolysate and coconut milk, which could be induced to form multiple shoots on transfer to a medium lacking casein hydrolysate, coconut milk and auxin. Rooting of isolated shoots by treatment with an auxin mixture (indole-3-acetic acid, indole-3-butyric acid and indole-3-propionic acid) and transfer of the plantlets to field have also been successful.

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Authors and Affiliations

  1. Biochemistry Division, National Chemical Laboratory, 411 008, Poona, India

    M. Kotwal Mridula, P. K. Gupta & A. F. Mascarenhas

Authors
  1. M. Kotwal Mridula
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  2. P. K. Gupta
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  3. A. F. Mascarenhas
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Additional information

NCL Communication No. 3122.

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Mridula, M.K., Gupta, P.K. & Mascarenhas, A.F. Rapid multiplication of Sapium sebiferum Roxb. by tissue culture. Plant Cell Tiss Organ Cult 2, 133–139 (1983). https://doi.org/10.1007/BF00043358

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  • DOI: https://doi.org/10.1007/BF00043358

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