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Callus culture of Coronilla varia L. (crownvetch): plant regeneration through somatic embryogenesis

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Abstract

Callus culture and plant regeneration through somatic embryogenesis have been obtained in Coronilla varia. Media used were UM (25) supplemented with 2 mg/l 2,4-D followed by subculture on MS (18) containing 1 mg/l 2-iP and 0.1 mg/l IAA. Embryoids developed into complete plantlets on filter paper saturated with hormone-free MS medium.

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Authors and Affiliations

  1. Facoltà di Agraria, Centro di Studio per il Miglioramento Genetico delle Piante Foraggere, Borgo XX Giugno, 06100, Perugia, Italy

    Domenico Mariotti & Sergio Arcioni

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  1. Domenico Mariotti
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  2. Sergio Arcioni
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Mariotti, D., Arcioni, S. Callus culture of Coronilla varia L. (crownvetch): plant regeneration through somatic embryogenesis. Plant Cell Tiss Organ Cult 2, 103–110 (1983). https://doi.org/10.1007/BF00043355

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  • DOI: https://doi.org/10.1007/BF00043355

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