Skip to main content
Log in

Micropropagation of Sesbania aculeata (Pers.) by adventitious organogenesis

  • Micropropagation Note
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Multiple shoots differentiated from hypocotyl explants of Sesbania aculeata (Pers.) syn S. cannabina (Retz.) Pers., a leguminous woody shrub, when cultured on Murashige and Skoog's basal medium supplemented with auxin (IBA, NAA) or auxin and cytokinin (IBA + BAP, NAA + BAP). Shoot budding occurred directly from the explant as well as from callus. Differentiation of shoot and root occurred in one step in the same concentration of auxin or auxin and cytokinin. Elongation of shoots occurred in the shoot induction medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ambasta SP (Ed) (1986) The useful plants of India. C.S.I.R., New Delhi

    Google Scholar 

  • Flick CE, Evans DA & Sharp WR (1983) Organogenesis. In: Evans DA, Sharp WR, Ammirato PV & Yamada Y (Eds) Handbook of Plant Cell Cultures, Vol 1 (pp 13–81). Macmillan Publishing Co., New York

    Google Scholar 

  • Hammatt N, Ghose TK & Davey MR (1986) Regeneration in legumes. In: Vasil IK (Ed) Cell Culture and Somatic Cell Genetics of Plants, Vol 3 (pp 67–95). Academic Press Inc., USA

    Google Scholar 

  • Kapoor S & Gupta SC (1986) Rapid in vitro differentiation of Sesbania bispinosa plants-a leguminous shrub. Plant Cell Tiss. Org. Cult. 7: 263–268

    Google Scholar 

  • Kartha KK, Pahl K, Leung NL & Mroginski LA (1981) Plant regeneration from meristems of grain legumes: soybean, cowpea, peanut, chickpea and bean. Can. J. Bot. 59: 1671–1679

    Google Scholar 

  • Khattar S & Mohan Ram HY (1982) Organogenesis in the cultured tissues of Sesbania sesban, a leguminous shrub. Indian J. Exp. Biol. 20: 216–219

    Google Scholar 

  • Khattar S & Mohan Ram HY (1983) Organogenesis and plantlet formation in vitro in Sesbania grandiflora (L) pers. Indian J. Exp. Biol. 21: 251–253

    Google Scholar 

  • Mathews H, Rao PS & Bhatia CR (1988) Increased frequency of normal plant regeneration from crown gall callus of Sesbania rostrata. Curr. Sci. 57: 1304–1306

    Google Scholar 

  • Mohan Ram HY & Kakkar M (1987) Utility of plant cell and tissue culture in the improvement of legumes in developing countries. In: Natsh S, Chopra VL & Ramchandran S (Eds) Biotechnology in Agriculture (pp 145–153). Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi

    Google Scholar 

  • Mroginski LA & Kartha KK (1984) Tissue culture of legumes for crop improvement. In: Janick J (Ed) Plant Breeding Reviews, Vol 2 (pp 215–264). AVI Publishing Co. Inc, West Port, Ct, USA

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Pandey P (1991) Plant regeneration from different explants in some members of Leguminosae. PhD thesis, R.D. University, Jabalpur (M.P.) India

  • Pandey P & Bansal YK (1992) Plant regeneration from leaf and hypocotyl explants of Glycine wightii (W and A) verdc var longicauda. Jap. Jour. Breed. 42 (1): 1–5

    Google Scholar 

  • Xu Z-H, Yank L-J, Wei Z-M & Gao M-X (1984) Plant regeneration in tissue cultures of four leguminous species. Acta Biol. Expl. Sin. 17: 483–486

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bansal, Y.K., Pandey, P. Micropropagation of Sesbania aculeata (Pers.) by adventitious organogenesis. Plant Cell Tiss Organ Cult 32, 351–355 (1993). https://doi.org/10.1007/BF00042299

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00042299

Key words

Navigation