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Plant regeneration from protoplasts isolated from cell suspension cultures of the wild tomato, Lycopersicon chilense Dun.

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Abstract

A protocol has been established for rapid, high frequency plant regeneration from protoplasts of the wild tomato species Lycopersicon chilense Dun. Cell suspension cultures were obtained from calli initiated from seedling stem explants. Protoplasts were isolated from cell suspensions by an overnight one-step enzyme digestion, purified by washing in salts solution and cultured in liquid medium. Dilution of liquid medium every 3 days, with medium containing low levels of growth regulators and sucrose, was critical for sustained colony formation. Up to 70% of protoplast-derived calli regenerated shoots when cultured on agar-solidified medium with Murashige & Skoog (1962) salts and vitamins, 2.0 mg l-1 zeatin and 0.1 mg l-1 indole acetic acid for 21 days, followed by transfer to the same medium lacking indole acetic acid.

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Abbreviations

BAP:

6-benzylaminopurine

IAA:

indole acetic acid

IBA:

indole butyric acid

MES-2:

(N-morpholino)-ethane sulfonic acid

NAA:

α-naphthaleneacetic acid

2,4-d :

2,4-dichlorophenoxyacetic acid

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Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK

    M. R. Davey & J. B. Power

  2. Tissue Culture Programme, National Agricultural Research Centre, Islamabad, Pakistan

    M. Latif & N. Mumtaz

Authors
  1. M. Latif
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  2. N. Mumtaz
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  3. M. R. Davey
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  4. J. B. Power
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Latif, M., Mumtaz, N., Davey, M.R. et al. Plant regeneration from protoplasts isolated from cell suspension cultures of the wild tomato, Lycopersicon chilense Dun.. Plant Cell Tiss Organ Cult 32, 311–317 (1993). https://doi.org/10.1007/BF00042294

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  • DOI: https://doi.org/10.1007/BF00042294

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