Abstract
Trials were conducted to study the effect of different photoperiods on in vitro growth of Mr.S.2/5 plum rootstock. Three photoperiods-16 h (control), 12 h and 8 h-were applied, with a PAR of 39 μmol m-2 sec-1. Tips collected from in vitro established shoots were used;ggrowth medium was MS with BA at 2.7 μM, GA3 at 0.7 μM and IBA at 0.3 μM.
Shoot proliferation after 45 days of growth was not statistically different between 12 h and 16 h of light, while the 8-h photoperiod gave a much lower rate of shoot formation. Shoot quality did not vary noticeably among the three treatments. Chlorophyll content and leaf thickness were not significantly modified even by a 50% reduction in light application. Stomata number and opening increased under 12 h of light.
Rooting percentage was reduced by shortening the photoperiod to 8 h while root length was positively influenced with a 12-h photoperiod.
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Abbreviations
- BA:
-
benzyladenine
- GA:
-
gibberellic acid
- IBA:
-
indolebutyric acid
References
Bressan PH, Kim YJ, Hyndman SE, Hasegawa PM & Bressan RA (1982) Factors affecting in vitro propagation of rose. J. Am. Soc. Hortic. Sci. 107: 979–990
Debergn P & Maene L (1977) Rapid clonal propagation of pathogen-free pelargonium plants starting from shoot tips and apical meristem. Acta Hortic. 78: 449–454
Economou AS & Read PE (1986) Influence of light duration and irradiance on micropropagation of a hardy deciduous azalea. J. Am. Soc. Hortic. Sci. 111: 146–149
Economou AS & Read PE (1987) Light treatments to improve efficiency of in vitro propagation system. HortScience 22: 751–754
Haramaki C (1971) Tissue culture of Gioxinia. Comb. Proc. Int. Plant Prop. Soc. 21: 442–448
Hasegawa PM, Murashige T & Takatori FH (1973) Propagation of asparagus through shoot apex culture: II Light and temperature requirements, transplantability of plants and cyto-histological characteristics. J. Amer. Soc. Hortic. Sci. 98: 143–148
Moran R (1982) Formulae for determination of chlorophyllous pigments extracted with N,N-Dimethylformamide. Plant Physiol. 69: 1376–1381
Murashige T & Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Yae BW, Zimmerman RH, Fordham I & Ko KC (1987) Influence of photoperiod, apical meristem and explant orientation on axillary shoot proliferation of apple cultivars in vitro. J. Am. Soc. Hortic. Sci. 112: 588–592
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Morini, S., Trinci, M. & Zacchini, M. Effect of different photoperiods on in vitro growth of Mr.S.2/5 plum rootstock. Plant Cell Tiss Organ Cult 25, 141–145 (1991). https://doi.org/10.1007/BF00042185
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DOI: https://doi.org/10.1007/BF00042185