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Effect of phloroglucinol on rooting and in vitro acclimatization of papaya (Carica papaya L. var. Maradol Roja)

  • Plant Tissue Culture
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Abstract

The development of somatic embryogenesis in papaya (Carica papaya L.) on semisolid culture medium provides an alternative for propagation and breeding of this species. However, a critical factor in the protocol is the low survival of plants in ex vitro conditions. The aim of this study was to determine the effect of the product phloroglucinol on rooting and in vitro acclimatization of somatic embryo-derived papaya shoots using sterile zeolite. In vitro shoots were placed in culture vessels with phloroglucinol at 0, 79, 118.5, or 158 μM with 9.8 μM of indole-3-butyric acid and zeolite as a support on half-strength Murashige and Skoog semi-solid medium. Different morphological and physiological variables were evaluated after 27 d of culture. In the treatments with phloroglucinol, very good rooting development in shoots from somatic embryogenesis was achieved. The phloroglucinol stimulated the root elongation and formation of new roots with respect to the agar control. The best results for in vitro shoots were obtained with 79 μM phloroglucinol plus 9.8-μM indole-3-butyric acid, where 100% rooting, with 2.40-cm root length, 1.76 root number and the highest values of photosynthesis (6.497 μmol CO2 m−2 s−1) was achieved in 27 d of culture compared to the control. This facilitated a better survival rate state of plants (96.5%) in ex vitro acclimatization conditions.

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References

  • Afreen F, Zobayed SMA, Kozai T (2002) Photoautotrophic culture of Coffea arabusta somatic embryos II: development of a bioreactor for the large-scale plantlet conversion from cotyledonary embryos. Ann Bot 9:20–29

    Google Scholar 

  • Agnihotri S, Singh SK, Jain M, Sharma M, Sharma AK, Chaturvedi HC (2004) In vitro cloning of female and male Carica papaya through tip of shoots and inflorescences. Indian J Biotechnol 3:235–240

    Google Scholar 

  • Arrieta-Ramos BG, Villegas-Monter A, Hernández-Bautista A, Rodríguez-Mendoza M, Ruiz-Posada L, García-Villanueva E (2010) Stomata and vigor of Valencia orange grafted on tolerant to citrus tristeza virus rootstocks. J Fitotec Mex 33:257–263

    Google Scholar 

  • Ascencio-Cabral A, Rodríguez B, Gutiérrez H, Gutiérrez A (2008) Plant regeneration of Carica papaya through somatic embryogenesis in response to light quality, gelling agent and phloridzin. Sci Hortic 118:155–160

    Article  CAS  Google Scholar 

  • Banergee J (2002) Tissue culture and transformation studies in Indian cultivars of papaya (Carica papaya L.) Thesis for the Degree of Doctor of Philosophy. University of Pune. India, pp 110

  • Barry-Etienne D, Bertrand B, Schlönvoigta A, Etienne H (2002) The morphological variability within a population of coffee somatic embryos produced in a bioreactor, affects the regeneration and the development of plants in nursery. Plant Cell Tissue Organ Cult 68:153–162

    Article  Google Scholar 

  • Cardona Ayala C, Araméndiz TH, Barrera CC (2009) Estimation of leaf area of papaya (Carica papaya L.) based on non-destructive sampling. UDCA News J Pop Sci 12:131–139

    Google Scholar 

  • Daud N, Faizal A, Greelen D (2013) Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light. In Vitro Cell Dev-Plant 49:183–190

    Article  CAS  Google Scholar 

  • De Klerk GJ, Guan HY, Huiman P, Marinova S (2011) Effects of phenolic compounds on adventitious root formation and oxidative decarboxylation of applied indoleacetic acid in Malus Jork 9. Plant Growth Regul 63:175–185

    Article  CAS  Google Scholar 

  • Dobránszki J, Teixeira da Silva JA (2010) Micropropagation of apple-a review. Biotechnol Adv 28:301–322

    Article  Google Scholar 

  • Farzana ARF, Palkadapala PGV, Meddegoda KMM, Samarajeewa PK, Eeswara JP (2008) Somatic embryogenesis in papaya (Carica papaya L. cv. Rathna). J Nat Sci Found Sri Lanka 36:41–50

    Google Scholar 

  • Fitch MM, Leong T, Akashi L, Yeh A, White S, De la Cruz A, Santo L, Ferreira S, Moore PH (2005) Growth and yield of clonally propagated and seedling-derived papayas I. growth. II yield. J Hortic Sci Biotechnol 40(5):1283–1290

    Google Scholar 

  • Hammatt N (1994) Promotion by phloroglucinol of adventitious root formation in micropropagated shoots of adult wild cherry (Prunus avium L.). Plant Growth Regul 14:127–132

    Article  CAS  Google Scholar 

  • Hazarika B (2006) Morpho-physiological disorders in in vitro culture of plant. Sci Hortic-Amst 108:105–120

    Article  CAS  Google Scholar 

  • Hofman P, Haisel D, Komenda J, Vagner M, Tichá I, Schäfer C, Čapková V (2002) Impact of in vitro cultivation conditions on stress responses and on changes in thylakoid membrane proteins and pigments of tobacco during ex vitro acclimation. Biol Plant 45:189–195

    Article  CAS  Google Scholar 

  • Husain MK, Anis M, Shahzad A (2008) In vitro propagation of a multipurpose leguminous tree (Pterocarpus marsupium Roxb.) using nodal explants. Acta Physiol Plant 30:353–359

    Article  CAS  Google Scholar 

  • Iarema L, Ferreira de Cruz AC, Witt CS, Carnevalli LLD, Fontes RV, Jardim de Oliveira E, Capos WO (2012) Photoautotrofic propagation of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]. Plant Cell Tiss Org 110:227–238

    Article  Google Scholar 

  • Jalal K, Shamsuddin A, Rahman M, Nurzatul N, Rozihan M (2013) Growth and total carotenoid, chlorophyll a and chlorophyll b of tropical microalgae (Isochrysis sp.) in laboratory cultured conditions. J Biol Sci 13:10–17

    Article  CAS  Google Scholar 

  • Kozai T, Xiao Y, Nguyen QT, Afreen F, Zobayed SMA (2005) Photoautotrophic (sugar-free medium) micropropagation system for large-scale commercialization. Propag Ornam Plants 5:23–34

    Google Scholar 

  • Kozai T, Zobayed SMA (2000) Acclimatization. In: Spier R (ed) Encyclopedia of cell technology. Wiley, New York, pp 1–12

    Google Scholar 

  • Larqué-Saavedra A, Trejo C (1988) Water in plants In: Handbook of Plant Physiology Practice. Trillas Publisher, Mexico, pp 88

  • Malabadi RB, Kumar SV, Mulgund GS, Nataraja K (2011) Induction of somatic embryogenesis in papaya (Carica papaya L.). Res Biotechnol 2:40–55

    Google Scholar 

  • Modgil M, Sharma DR, Bhardwaj SV (1999) Micropropagation of apple cv. Tydeman Early Worcester. Sci Hortic-Amst 81:179–188

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nzilani NM, Karambu FR, Edward GM, Wanjiru AK (2013) In vitro regeneration of selected Kenyan papaya (Carica papaya L.) lines through shoot tip culture. Afr J Biotechnol 12:6826–6832

    Google Scholar 

  • Posada-Pérez L, Gómez-Kosky R, Reyes VM (2007) Somatic embryogenesis in Carica papaya L. var. Maradol Roja. Biotecnol Veg 7(3):131–138

    Google Scholar 

  • Pospisilova J, Synkova H, Haisel D, Semoradova S (2007) Acclimatization of plantles to ex vitro conditions: effects of air, humidity, irradiance, CO2 concentration and abscisic acid. Acta Hortic 748:29–39

    Article  CAS  Google Scholar 

  • Romais E, Teixeira C, Ribeiro E, Lopes S (2000) Efeito do floroglucinol na reaçao morfogênica in vitro de segmentos internodais de Citrus sinensis (L.) Osbeck cv Pera. Rev Ceres 47:113–120

    Google Scholar 

  • Rosell M, Galloso R, Calvo B (2006) Zeolite as an active mineral additive in high performance concrete. Geol Bull 117:63–67

    Google Scholar 

  • Ross S, Castillo A (2009) Mass propagation of Vaccinium corymbosum in bioreactors. Agrociencia XIII(2):1–18

    Google Scholar 

  • Ross S, Castillo A (2010) Micropropagation of Achyrocline flaccida (Weinm) DC. in liquid culture media. Agrociencia XIV(1):1–7

    Google Scholar 

  • Ross S, Grasso R (2010) In vitro propagation of ‘Guayabo del país’ (Acca sellowiana (Berg.) Burret). Fruit Veg Cereal Sci Biotechnol 4(special issue 1):83–87

    Google Scholar 

  • Sáez P, Bravo L, Sáez K, Sánchez-Olate M, Latsegue M, Ríos D (2012) Photosynthetic and leaf anatomical characteristics of Castanea sativa: a comparison between in vitro and nursery plants. Biol Plant 36:15–24

    Article  Google Scholar 

  • Sekeli R, Abdullah JO, Namasivayam P, Muda P, Abu Bakar UM (2013) Better rooting procedure to enhance survival rate of field grown Malaysian Eksotika papaya transformed with 1-Aminocyclopropane-1-carboxylic acid oxidase gene. ISRN Biotechnol 13:1–10

    Article  Google Scholar 

  • Sharma M, Modgil M, Sharma DR (2000) Successful propagation in vitro of apple rootstock MM106 and influence of phloroglucinol. Indian J Exp Biol 38:1236–1240

    CAS  PubMed  Google Scholar 

  • Stirban M (1985) Procese primare ĩn fotosintezã. Didact Ed. Si Pedag. Bucharest. pp 229

  • Teixeira da Silva JA (2013) In vitro rhizogenesis in papaya (Carica papaya L.). J Plant Dev 20:51–53

    Google Scholar 

  • Teixeira da Silva JA (2014) Photoauto-, Photohetero-and Photomixotrophic in vitro propagation of papaya (Carica papaya L.) and response of seed and seedlings to light-emitting diodes. Thammasat Int J Sci Technol 19:57–71

    Google Scholar 

  • Teixeira da Silva JA, Dobránszki J, Ross S (2013) Phloroglucinol in plant tissue culture. In Vitro Cell Dev-Plant 49:1–16

    Article  CAS  Google Scholar 

  • Webster CA, Jones OP (1989) Micropropagation of apple rootstock M9: effect of sustained subculture on apparent rejuvenation in vitro. J Hortic Sci 64:421–428

    Article  Google Scholar 

  • Ziv M, Meir G, Halevy AH (1983) Factors influencing the production of hardened glaucous carnation plantlets in vitro. Plant Cell Tiss Org 2:55–56

    Article  Google Scholar 

Download references

Acknowledgment

We are also grateful to Emer. Prof. Dr. ir. Oswald Van Cleemput, Ghent University, Belgium, for his kind help in revising the English language.

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Authors and Affiliations

  1. Instituto de Biotecnología de las Plantas, Universidad Central “Marta Abreu” de Las Villas, Carretera a Camajuaní Km 5 ½, Santa Clara, Villa Clara, Cuba

    Laisyn Posada Pérez, Yenny Padrón Montesinos, Raúl Barbón Rodriguez & Rafael Gómez-Kosky

  2. Centro de Bioplantas, Universidad de Ciego de Ávila, Carretera a Morón km 9, Ciego de Ávila, Cuba

    Justo González Olmedo, Romelio Rodríguez Sánchez & Rene Carlos Rodriguez Escriba

  3. Centro de Bioactivos Químicos, Universidad Central “Marta Abreu” de Las Villas, Carretera a Camajuaní Km 5 ½, Santa Clara, Villa Clara, Cuba

    Osvaldo Norman Montenegro

  4. University of Belize, Hummingbird Avenue, Belmopan, Belize, Central America

    Dion Daniels

Authors
  1. Laisyn Posada Pérez
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  2. Yenny Padrón Montesinos
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  3. Justo González Olmedo
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  4. Raúl Barbón Rodriguez
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  5. Romelio Rodríguez Sánchez
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  6. Osvaldo Norman Montenegro
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  7. Rene Carlos Rodriguez Escriba
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  8. Dion Daniels
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  9. Rafael Gómez-Kosky
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Correspondence to Rafael Gómez-Kosky.

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Editor: Ewen Mullins

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Pérez, L.P., Montesinos, Y.P., Olmedo, J.G. et al. Effect of phloroglucinol on rooting and in vitro acclimatization of papaya (Carica papaya L. var. Maradol Roja). In Vitro Cell.Dev.Biol.-Plant 52, 196–203 (2016). https://doi.org/10.1007/s11627-015-9733-6

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  • DOI: https://doi.org/10.1007/s11627-015-9733-6

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