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Isolation of protoplasts from different Eucalyptus species and preliminary studies on regeneration

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Abstract

Protoplasts were isolated from different Eucalyptus clones and hybrids using mesophyll tissue, calli and cell suspension cultures. The protoplast yields differed greatly according to the starting material and adaptations of the basic procedure had to be designed in specific cases.

Eucalyptus protoplasts are representative of recalcitrant woody plant systems since their proliferation is limited in culture. The best results were obtained with protoplasts from cell suspension cultures. A screening of factors increasing proliferation was performed. When some of these factors were combined the cell division frequency was enhanced and microcalli were obtained.

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Abbreviations

B.A.P.:

Benzylaminopurine

2-4D:

2-4 dichlorophenoxy-acetic acid

F.D.A.:

Fluorescein diacetate

F.W.:

Fresh weight

M.E.S.:

Morpholino ethane sulfonic acid

M.S.:

Murashige & Skoog (1962)

N.A.A.:

Naphtalene acetic acid

TRIS:

Tri (hydroxymethyl amino methane)

V.KM.:

Medium-Kao and Michayluk medium modified by Vasil (Vasil & Vasil 1980)

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Authors and Affiliations

  1. Centre de Physiologie Végétale, Université Paul Sabatier, URA CNRS 241, 118 route de Narbonne, 31062, Toulouse Cédex, France

    C. Teullères & A. M. Boudet

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  1. C. Teullères
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  2. A. M. Boudet
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Teullères, C., Boudet, A.M. Isolation of protoplasts from different Eucalyptus species and preliminary studies on regeneration. Plant Cell Tiss Organ Cult 25, 133–140 (1991). https://doi.org/10.1007/BF00042184

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  • DOI: https://doi.org/10.1007/BF00042184

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