Abstract
Carrot cells were cultured under various light spectra and intensities at different times following the initiation of suspension cultures from callus. The highest intensity white and blue light treatments were inhibitory to growth and somatic embryogenesis. Red and green light were not different from dark treatments which produced the highest total number of embryoids. After extended time in culture, carrot cells in blue light produced secondary embryoids and anthocyanin. Cultures in red light had multiple cotyledons and orange-pigmented radicles. Leafy cotyledons occurred in all light treatments. Abscisic acid production peaked at the heart stage of embryogenesis and synthesis was most pronounced in blue light. Red light enhanced development to the heart stage. Both the red and blue light spectra may be used to manipulate carrot cell cultures to optimize growth.
Similar content being viewed by others
References
Ackerson RC (1984) Regulation of soybean embryogenesis by abscisic acid. J Exp Bot 35: 403–413.
Altmann A, Goren R (1971) Promotion of callus formation by abscisic acid in citrus bud cultures. Plant Physiol 47: 844–846.
Ammirato PV (1974) The effects of abscisic acid on the development of somatic embryos from cells of caraway (Carum caryi L.). Bot Gaz 135: 328–337.
Ammirato PV (1977) Hormonal control of somatic embryo development from cultured cells of caraway. Interactions of abscisic acid, zeatin, and gibberellic acid. Plant Physiol 59: 579–586.
Ammirato PV, Steward FC (1971) Some effects of environment on the development of embryos from cultured free cells. Bot Gaz 32: 149–158.
Beggs CJ, Holmes MG, Jabben M, Schafer E (1980) Action spectra for the inhibition of hypocotyl growth by continuous irradiation in light and dark-grown Sinapsis alba L. seedlings. Plant Physiol 66: 615–618.
Blumenfeld A, Gavit S (1970) Interaction of kinetin and abscisic acid in the growth of soybean callus. Plant Physiol 45: 535–536.
Finklestein RR, Tenbarge KM, Shumway JE, Crouch ML (1985) Role of ABA in maturation of rapeseed embryos. Plant Physiol 78: 630–636.
Fridborg G, Eriksson T (1975) Partial reversal by cytokinin and (2-chloroethyl)-trimethy-lammonium chloride of near-ultraviolet inhibited growth and morphogenesis in callus cultures. Physiol Plant 34: 162–166.
Fujimura T, Komamine A (1975) Effects of various growth regulators on the embryogenesis in a carrot cell suspension culture. Pl Sci Let 5: 359–364.
Gamborg OL, LaRue TAG (1971) Ethylene production by plant cell cultures: the effects of auxins, abscisic acid, and kinetin on ethylene production in suspension cultures of rose and Ruta cells. Plant Physiol 48: 399–401.
Halperin W (1966) Alternative morphogenetic events in cell suspensions. Amer J Bot 53: 443–453.
Halperin W (1970) Embryos from somatic plant cells. In HAPadykula [ed.], Control mechanisms in the expression of cellular phenotypes, pp. 169–191. Symp Int Soc Cell Biol, Acad Press, New York.
Hubick KT, Reid DM (1980) A rapid method for the extraction and analysis of abscisic acid from plant tissue. Plant Physiol 65: 523–525.
Kamada H, Harada H (1979) Studies on the organogenesis in carrot tissue cultures. I. Effects of growth regulators on somatic embryogenesis and root formation. Z Pflanzenphysiol 91: 255–266.
Kamada H, Harada H (1981) Changes in the endogenous level and effects of abscisic acid during somatic embryogenesis of Daucus carota L. Pl Cell Physiol 22: 1423–1429.
Klein RM (1964) Repression of tissue culture growth by visible and near-visible radiation. Plant Physiol 39: 536–539.
Kochba J, Spiegel-Roy P, Neumann H, Saad S (1978) Stimulation of embryogenesis in citrus ovular callus by ABA, ethephon, CCC, and alar and its suppression by GA3. Z Pflanzenphysiol 89: 427–432.
Krikorian AD (1982) Cloning higher plants from aseptically cultured tissues and cells. Biol Rev 57: 151–218.
Ku PK, Mancinelli AL (1972) Photocontrol of anthocyanin synthesis. Plant Physiol 49: 212–217.
Lavee S, Glenda M (1969) The effect of growth regulating substances and light on olive callus growth in vitro. J Exp Bot 20: 604–614.
Mancinelli AL (1985) Light-dependent anthocyanin synthesis: A model system for the study of plant photomorphogenesis. Bot Rev 51: 107–157.
Michler CH, Lineberger RD, Chism GW (1986) A highly sensitive method for quantitative determination of abscisic acid. Plant Physiol 82: 600–603.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.
Newcomb W, Wetherell DF (1970) The effects of 2,4,6-trichloro-phenoxyacetic acid on embryogenesis in wild carrot tissue cultures. Bot Gaz 131: 242–245.
Ozeki Y, Komamine A (1985) Effects of inoculum density, zeatin, and sucrose on anthocyanin accumulation in a carrot suspension culture. Plant cell Tissue Organ Culture 5: 45–53.
Rabino I, Mancinelli AL, Kuzmanoff KM (1977) Photocontrol of anthocyanin synthesis. VI. Spectral sensitivity, irradiance dependence and reciprocity relationships. Plant Physiol 59: 569–573.
Reinert J (1958) Morphogenese und ihre Kontrolle an Gewebekulturen aus Carotten. Naturwissenschaften 45: 344–345.
Seibert M, Wetherbee DJ, Job DD (1975) Effects of light intensity and spectral quality on growth and shoot initiation in tobacco callus. Plant Physiol 56: 130–139.
Smith SM, Street HE (1973) The decline of embryogenic potential as callus and suspension cultures of carrot (Daucus carota L.) are serially subcultured. Ann Bot 38: 223–241.
Steward FC, Mapes MO, Mears K (1958) Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. Amer J Bot 45: 705–708.
Thomas B, Dickinson HG (1979) Evidence for two photoreceptors controlling growth of de-etiolated seedlings. Planta 146: 545–550.
Tisserat B, Murashige T (1977) Repression of asexual embryogenesis in vitro by some plant regulators. In Vitro 13: 799–805.
Umbeck PF, Norstog K (1979) Effects of abscisic acid and ammonium ions on morphogenesis of a cultured barley embryos. Bull Torrey Bot Club 106; 110–116.
Umbeck PF (1981) Does ABA play a role in seed germination? Israel J Bot 29: 168–180.
Weis JS, Jaffe MJ (1969) Photoenhancement by blue light of organogenesis in tobacco pith cultures. Physiol Plant 22: 171–176.
Wetherell DF (1984) Enhanced adventive embryogenesis resulting from plasmolysis of cultured wild carrot cells. Plant Cell Tissue Organ Culture 3: 221–227.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Michler, C.H., Lineberger, R.D. Effects of light on somatic embryo development and abscisic levels in carrot suspension cultures. Plant Cell Tiss Organ Cult 11, 189–207 (1987). https://doi.org/10.1007/BF00040425
Issue Date:
DOI: https://doi.org/10.1007/BF00040425