Summary
Experimental conditions were established that resulted in high yields and good viability of the protoplasts obtained from leaves of Vitis vinifera, cv. Chardonnay regenerated in vitro by somatic embryogenesis. The effect of factors of the culture medium and various environmental conditions upon the frequency of cell division has been examined, and a method of culture is described by which protoplasts were induced to begin division. Most protoplasts obtained in this way regenerated cell walls within the first few days and cell division occurred after 10 days of culture in a liquid medium. Some cells have divided two or even three times. Nevertheless, the cells did not continue dividing beyond this stage.
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Barbier, M., Bessis, R. Isolation and culture of grape vine cv. Chardonnay leaf protoplasts. Euphytica 47, 39–44 (1990). https://doi.org/10.1007/BF00040361
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DOI: https://doi.org/10.1007/BF00040361