Abstract
Five different liquid medium culture methods for meristem propagation of bananas were investigated and compared with solid medium culture. Treatments studied were: gelled culture medium (treatment 1); liquid medium with immersion of the plants (treatment 2); liquid medium with cellulose culture support (treatment 3); liquid medium with partial immersion of the plants (treatment 4); liquid medium aerated by bubbling (treatment 5); liquid medium with temporary immersion of the explants for 20 min every 2h (treatment 6). After 20 days of culture, three culture groups with statistically different multiplication rates were observed:
-
-shoots in simple liquid medium and those on cellulose substrate proliferated little or not at all,
-
-shoots on gelled medium, those subjected to partial immersion and those in aerated medium displayed multiplication rates of 2.2 to 3.1, and
-
-the highest multiplication rate (>5) was observed in explants subjected to temporary immersion in the medium.
Two groups of treatments differed in the accumulation of dry matter: the smallest weight (around 0.5 g) was observed in treatment 1, 2, 3 and 4, and accumulation was 2 to 5 times greater in the explants in aerated liquid medium and those subjected to temporary immersion. The highest multiplication rates and weight gains were observed in aerated treatments (treatments 4 and 5). Shoots in liquid medium continuously aerated by bubbling displayed hyperhydricity of the outer leaf sheaths. This was not observed with temporary immersion of explants.
Similar content being viewed by others
References
Aitken-Christie, J (1991) Automation. In: Debergh, PC & Zimmerman, RH (Eds) Micropropagation: Technology and Application (pp 363–388). Kluwer Academic Publishers, Dordrecht
Aitken-Christie, J & Jones, C (1987) Towards automation: radiata pine shoot hedges in vitro. Plant Cell Tiss. Org. Cult. 8: 185–196
Debergh, PC (1983) Effects of agar brand and concentration on the tissue culture medium. Physiol Plant 59: 270–276
Gawel, NJ & Robacker, CD (1990) Somatic embryogenesis in two Gossypium hirsutum genotypes on semi-solid versus liquid proliferation media. Plant Cell Tiss. Org. Cult. 23: 201–204
Hammerschlag, F (1982) Factors affecting establishment and growth of peach shoots in vitro. HortScience 17: 85–86
Harris, RE & Mason, EBB (1983) Two machines for in vitro propagation of plants in liquid media. Can. J. Plant Sci. 63: 311–316
Jones, AM & Petolino, JF (1988) Effects of support medium on embryo and plant production from cultured anthers of soft-red winter wheat. Plant Cell Tiss. Org. Cult. 12: 253–261
Morel, G & Wetmore, RH (1951) Tissue culture of monocotyledons. Amer. J. Bot. 38: 138–140
Murashige, T & Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Newman, D (1939) The distribution of range in samples from normal population expressed in terms of an independent estimate of standard deviation. Biometrika 31: 20–30
Preil (1991) Application of bioreactors in plant propagation. In: Debergh, PC & Zimmerman, RH (Eds) Micropropagation: Technology and Application (pp 426–445). Kluwer Academic Publishers, Dordrecht
Skidmore, DI, Simon, AJ & Bedis (1988) In vitro culture of shoots of Pinus caribaea on a liquid medium. Plant Cell Tiss. Org. Cult. 14: 129–136
Snir, I & Erez, A (1980) In vitro propagation of Malling Merton apple rootstocks. HortScience 15: 597–598
Vuylsteke, DR (1989) Shoot-tip culture for the propagation, conservation and exchange of Musa germplasm. International Board for Plant Genetic Resources, Rome (pp 1–56). ISBN 92–9043–140–7
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Alvard, D., Cote, F. & Teisson, C. Comparison of methods of liquid medium culture for banana micropropagation. Plant Cell Tiss Organ Cult 32, 55–60 (1993). https://doi.org/10.1007/BF00040116
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00040116