Skip to main content
SpringerLink
Log in

Regeneration of plantlets from hypocotyl-derived callus of Coptis teeta

  • Micropropagation Note
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Callus cultures of Coptis teeta were established from hypocotyl segments (excised from aseptically germinating seeds) on Murashige and Skoog (MS) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin. Microshoots were produced within 6–7 weeks of subculturing this callus in 1/2 MS nutrient medium supplemented with kinetin alone. Excised microshoots were rooted in 1/2 MS nutrient medium containing indolebutyric acid (IBA). The complete plantlets were hardened and established.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Borkowska B & Opilowska M (1988) Influence of BA and other cytokinins on proliferation and metabolic status of sour cherry cultures cultivated in vitro. Fruit Sci Rep. 15: 147–156

    Google Scholar 

  • Hegde SN (1988) Coptis teeta Wall. (Mishmi teeta)-A rare medicinal plant from Arunachal Pradesh. Arunachal For. News. 6: 27–29

    Google Scholar 

  • Jain SK & Sastry ARK (1980) Threatened Plants of India, A State of the Art Report (pp 10 & 19). Botanical Survey of India, Howrah

    Google Scholar 

  • Mudgal V & Jain SK (1980) Coptis teeta Wall. local uses, distribution and cultivation. BOBSI 22: 1–4

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Nakagawa K, Miura Y, Fukin H & Tabata M (1982) Clonal propagation of medicinal plants through the induction of somatic embryogenesis from the cultured plant cell. In: Fujiwara A (Ed) Plant Tissue Culture (pp 701–702). Abe Photo Printing Co Ltd. Tokyo, Japan

    Google Scholar 

  • Sato F & Yamada Y (1984) High berberine producing cultures of Coptis japonica cells. Phytochemistry 23: 281–285

    Google Scholar 

  • Staba EJ (1977) Tissue culture and pharmacy. In: Reinert J & Bajaj YPS (Eds) Applied and Fundamental Aspects of Plant Cell Tissue and Organ Culture (pp 699–715). Springer-Verlag, Berlin, New York

    Google Scholar 

  • Syôno K & Furuya T (1972) The differentiation of Coptis plants in vitro from callus cultures. Experientia 28: 286

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, 793 014, Shillong, India

    Pramod Tandon & Tilok S Rathore

Authors
  1. Pramod Tandon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tilok S Rathore
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tandon, P., Rathore, T.S. Regeneration of plantlets from hypocotyl-derived callus of Coptis teeta . Plant Cell Tiss Organ Cult 28, 115–117 (1992). https://doi.org/10.1007/BF00039923

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00039923

Key words

Search

Navigation