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Somatic embryogenesis of agriculturally important lupin species (Lupinus angustifolius, L. albus, L. mutabilis)

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Abstract

Somatic embryos were obtained from immature cotyledons of Lupinus angustifolius, L. albus and L. mutabilis but not from L. luteus. Different kinds of basal media and plant growth regulators in primary and secondary culture were tested. The best induction media were based on B5 and were supplemented with 5 mg I-1 2,4-D alone or with 0.25 mg I-1 kinetin. Mature stage somatic embryos were obtained on media containing ABA (0.1–0.5 mg I-1) and a high NH4/NO3 ratio. Embryo germination and plantlet development occurred on MS media supplemented with glutamine or GA3.

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  1. Department of Plant Tissue Culture, Plant Breeding and Acclimatization Institute, Radzików, 05-870, Blonie, Poland

    Anna Nadolska-Orczyk

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  1. Anna Nadolska-Orczyk
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Nadolska-Orczyk, A. Somatic embryogenesis of agriculturally important lupin species (Lupinus angustifolius, L. albus, L. mutabilis). Plant Cell Tiss Organ Cult 28, 19–25 (1992). https://doi.org/10.1007/BF00039911

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  • DOI: https://doi.org/10.1007/BF00039911

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