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Oil palm (Elaeis guineensis) protoplasts: isolation, culture and microcallus formation

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Abstract

Procedures are deseribed for the efficient isolation of protoplasts from a variety of oil palm (Elaeis guineensis Jacq.) tissues. Various factors including donor source, composition of enzyme mixture and culture medium affected the yield and viability of the protoplasts Polyembryogenic cultures of oil palm were the most suitable starting material in terms of yield, viability and metabolic competence. Pectolyase Y-23 in association with cellulase and hemicellulase was required for the efficient release of protoplasts from the oil palm tissues. Limited cell division to form microcallus was observed at very low frequency (<0.01%) when glutathione and catalase were incorporated in the culture medium.

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Abbreviations

2,4-d :

dichlorophenoxyacetic acid

DTT:

dithiothreitol

MES:

2[N-morpholino] ethanesulphonic acid

NAA:

1-naphthalene acetic acid

PVP:

polyvinylpyrrolidone

References

  • Abdullah R, Cocking EC & Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/technology 4: 1087–1090

    Google Scholar 

  • Ahmad KZ & Sagi F (1993) Culture of and fertile plant regeneration from regenerable embryogenic suspension cell-derived protoplasts of wheat (Triticum aestivum L.). Plant Cell Rep. 12: 175–179

    Google Scholar 

  • Bass A & Hughes W (1984) Conditions for the isolation and regeneration of viable protoplasts of oil palm. Plant Cell Rep. 3: 169–171

    Google Scholar 

  • Brackpool AL, Branton BL & Blake J (1986) Regeneration in palms. In: Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants, Vol 3 (pp 207–222). Academic Press, Orlando

    Google Scholar 

  • Carswell GK, Johnson CM, Shillite RD & Harms CT (1989) Oacetyl-salicylic acid promotes colony formation from protoplasts of an clite maize inbred. Plant Cell Rep. 8: 282–284

    Google Scholar 

  • Donn G, Nilges M & Moroez S (1990) Stable transformation of maize with a chimaeric, modified phosphinotrycin-acyltransferase gene from Streptomyces viridochromogenes. In: Nijkamp HJJ, Van derPlas LHW & Aartrijk J (ed) Progress in Plant Cellular and Molecular Biology, Abstr. A2–38 (p. 53). Kluwer Publicatians, Dordrecht

    Google Scholar 

  • Gamborg OL, Miller RA & Ojima K (1968) Plant cell cultures 1. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158

    Google Scholar 

  • Ghosh Biswas GC, Burkhardt PK, Wann J, Kloti A & Potrykus I (1994) Fertile Indica rice plants regenerated from protaplasts isolated from scutellar tissue of immature embryos. Plant Cell Rep. 13: 528–532

    Google Scholar 

  • Ishii S (1989) Factors influencing protoplast viability of suspension cultured rice cells during isolation process. Plant Physiol. 88: 26–29

    Google Scholar 

  • Jain RK, Khehra GS, Lee SH, Blackhall NW, Marchant R, Davey MR, Power JB & Cocking EC (1995) An improved procedure for plant regeneration from indica and japonica rice protoplast. Plant Cell Rep. 14: 515–519

    Google Scholar 

  • Kao KN (1982) Plant protaplast fusion and isolation of heterokaryocytes. In: Wetter LR & Constable F (ed) Plant Tissue Culture Methods (2nd edition, pp 49–52). National Research Council of Canada, Prairie Regional Laboratory, Saskatoon, Saskatchewan. NRCC 19876.

    Google Scholar 

  • Kao KN & Michayluk MK (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126: 105–110

    Google Scholar 

  • Li ZY, Xia GM, Chen HM & Guo GQ (1992) Plant regeneration from protoplasts derived from embryogenesis suspension cultures of wheat (Triticum aestivum L.). Plant Physiol. 139: 714–718

    Google Scholar 

  • Megia R, Haicour R, Rossignol L & Sihachakr D (1992) Callus formation from cultured protoplasts of banana (Musa sp). Plant Sci. 85: 91–98

    Google Scholar 

  • Mielke T (1995) Oil World Statistics Update. In: Mielke T (ed). Oil World (pp 55–56). ISTA Mielke GmbH, Hamburg

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioyassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  • Nagata & Takebe I (1970) Cell wall regeneration by isolated tomato fruit protoplasts. Protoplasma 64: 460–480

    Google Scholar 

  • Paranjothy K, Rohani O, Tarmizi AH, Tan CS & Tan CC (1989) Current status and strategies of oil palm tissue culture research. Proceedings of the 1989 PORIM International Palm Oil Development Conference (Agriculture) (pp 109–121)

  • Paranjothy K (1982) A review of tissue culture of oil palm and other palms. PORIM Occasional Paper 3: 1–22

    Google Scholar 

  • Peri A, Aviv D & Galun E (1988) Ethylene and in vitro culture of potato: suppression of ethylene generation vastly improves protoplast yield, plating efficiency and transient expression of an alien gene. Plant Cell Rep. 7: 403–406

    Google Scholar 

  • Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D & Detmer JJ (1989) Genetically transformed maize plants from protoplasts. Science 240: 204–207

    Google Scholar 

  • Russell JA & McCown BH (1986) Culture and regeneration of populus leaf protoplasts isolated from non-seedling tissue. Plant Science 46: 133–142

    Google Scholar 

  • Sambanthamurthi R, Oo KC & Ong ASH (1987) Lipid metabolism in oil palm (Elaeis guineensis and Elacis oleifera) protoplasts. Plant Sci. 51: 97–103

    Google Scholar 

  • Shillito RD, Carswell GK, Johnson CM, DiMaio JJ & Harms CT (1989) Regeneration of fertile plants from protoplasts of elite inbred maize. Bio/Technol. 7: 581–587

    Google Scholar 

  • Teixeira JB, Sondahl MR, Nakamura T & Kirby EG (1995) Establishment of oil palm cell suspensions and plant regeneration. Plant Cell Tiss. Org. Cult. 40: 105–111

    Google Scholar 

  • Toriyama K, Arimoto Y, Lichimiyia H & Hinata K (1988) Transgenic rice plants after direct gene transfer into protoplasts. Bio/Technol. 6: 1072–1074

    Google Scholar 

  • Touchet BD, Duval Y & Pannetier C (1991) Plant regeneration from embryogenic suspension cultures of oil palm (Elaeis guineensis Jacq.). Plant Cell Rep. 10: 529–532

    Google Scholar 

  • Wang XH & Lorz H (1994) Plant regeneration from protoplasts of wild barley (Hordeum murinum L.). Plant Cell Rep. 13: 139–144

    Google Scholar 

  • Zhang J, Tiwari VK, Golds TJ, Blackhall NW, Cocking EC, Mulligen BJ, Power JB & Davey MR (1995) Parameters influencing transient and stable transformation of barley (Hordeum vulgare L.) protoplasts. Plant Cell Tiss. Org. Cult. 41: 125–138

    Google Scholar 

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Authors and Affiliations

  1. Plant Science and Biotechnology Unit, Palm Oil Research Institute of Malaysia, P.O. Box 10620, 50720, Kuala Lumpur, Malaysia

    Ravigadevi Sambanthamurthi, Siti Hasnah Parman & Mohd. Roslan Md. Noor

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  1. Ravigadevi Sambanthamurthi
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  2. Siti Hasnah Parman
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  3. Mohd. Roslan Md. Noor
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Sambanthamurthi, R., Parman, S.H. & Noor, M.R.M. Oil palm (Elaeis guineensis) protoplasts: isolation, culture and microcallus formation. Plant Cell Tiss Organ Cult 46, 35–41 (1996). https://doi.org/10.1007/BF00039694

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  • DOI: https://doi.org/10.1007/BF00039694

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