Summary
Fusarium solani f.sp. eumartii Carp. Snyder and Hansen (Fusarium eumartii) is a soil inhabitant that induces the so-called Potato Wilt and Stem End Rot disease. Prior to wilting, the pathogen induces peculiar small bronze spots on the leaflets. Failure to isolate F. eumartii from infected leaflets suggests the involvement of a toxin in the disease. The fungus was grown in liquid Richard's medium and thereafter a filtrate was obtained dialyzing (MW cutoff 12,000–14,000) and sterilizing the culture by filtration (0.22 μm). Potato leaves treated with both the pathogen or the filtrate showed symptoms of bronze spots and significantly higher electrolyte leakage when compared to controls. Tomato leaves showed neither bronze spots nor electrolyte leakage after plant inoculation with the pathogen or with the filtrate treatment. Both, the absence of visible symptoms and the lack of electrolyte leakage in tomato could be associated to a certain degree of host specificity of the F. eumartii filtrate towards potato. The filtrate also induced symptoms similar to infections by F. eumartii in adult plants and in vitro plantlets of cultivars Huinkul MAG and Kennebec. Callus responses to the filtrate were related to responses of the cultivars to the pathogen in greenhouse. These results show the potential of the culture filtrate of F. eumartii for use in screening for wilting resistance.
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References
Arcieni, S., M., Pezzotti & F., Damiani, 1987. In vitro selection of alfalfa plants resistant to Fusarium oxysporum f.sp. medicaginis. Theoretical Applied Genetics 74: 700–705.
Botta, G.L., 1992. Marchitamiento y punta seca de la papa. Fusarium solani f.sp. eumartii Carp. Snyder and Hansen. I. Selección de un método de inoculación in vitro para la evaluación del comportamiento de cultivares de papa. II. Evaluación de la variación somaclonal como potencial inductor de resistencia. Magister Scientae Tesis. 95 pp.
Calderoni, A., 1954. Comportamiento frente al marchitamiento o 084 fusariosis (Fusarium solani var. eumartii) en 60 variedades y clones de papa. Segunda Reunión de la Papa, Ministerio de Agricultura y Ganadería de la Nación, Buenos Aires. pp. 44–47.
Calderoni, A., 1978. Fusariosis, Marchitamiento o Punta Seca. In: Enfermedades de la Papa y su Control, pp. 39–44. Hemisferio 084 Sur, Argentina.
Carrera, C.J.M., 1972. El género Fusarium en la República Argentina Sistemática y consideraciones sobre las especies causales de enfermedades en las plantas. Revista de Investigaciones Agropecuarias, INTA 9: 41–100.
Daub, M., 1986. Tissue culture and the selection of resistance to pathogens. Annual Review of Phytopathology 24: 159–186.
Escande, A. & W., Radtke, 1973. Comparación de métodos de inoculación con Fusarium eumartii en cultivares de papa. Revista de Investigaciones Agropecuarias, INTA 10: 212–233.
Escande, A.R., A.V., Calderoni & A.L., Melegari, 1984. Marchitamiento y punta seca. In: INTA, FCA-UNMDP & CIAM (Eds). La papa: diagnóstico y control de sus enfermedades, pp. 15–16. 084 Rohm & Haas, Rosario.
Frame, B., Y., Kang-Fu, B.R., Christie & K.P., Pauls, 1991. In vitro selection for resistance to Verticillium wilt in alfalfa (Medicago sativa L.) using a fungal culture filtrate. Physiological and Molecular Plant Pathology 39: 325–340.
Gerlach, W. & H., Nirenberg, 1982. Fusarium eumartii Carpenter. In: P., Parey (Ed.), A Pictorial Atlas, pp. 373–376. Biologische Bundesanstalt für Land- und Forstwirtschaft Institut für Mikrobiologie, Berlin-Dahlem.
Goss, R.W., 1924. Potato wilt and stem-end rot caused by Fusarium eumartii. University of Nebraska. Agricultural Experiment Station. Research Bulletin 27. USA.
Hooker, W.J., 1980. Compendio de Enfermedades de Papa. Centro 084 Internacional de la Papa, Lima, Perú.
Iacobellis, N.S. & A., Bottalico, 1981. Effects of some trichothecenes produced by species of Fusarium on electrolyte leakage from tomato leaf discs. Phytopathologia Mediterranea 20: 129–132.
Malamud, O.S., 1970. Incidencia de la fusariosis en el rendimiento de la papa. Estación Experimental Regional Agropecuaria. Balcarce. IPE No 16.
Misaghi, I.J., 1982. Physiology and Biochemistry of Plant Pathogen Interactions. Plenum Press, New York.
Murashige, T. & F., Skoog, 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant 15: 473–497.
Ochatt, S.J. & O.H., Caso, 1986. Differential requirements among tissue source in Solanum tuberosum L. ssp. andigena callus culture. Turrialba Vol. 36 No 3: 363–368.
Sukumaran, N.P. & C.J., Weiser, 1972. An excised leaflet test for evaluating potato frost tolerance. Hort. Science 7: 467–468.
The Commonwealth Mycological Institute, 1985. Medios de cultivo y métodos micológicos. In: Oficina Regional de la FAO para América Latina y el Caribe (Ed.). Manual para patólogos vegetales, 084 pp. 393–413. Pacific Press, Lima.
Thomas, C.A., 1949. A wilt inducing polysaccharide from Fusarium solani f.sp. eumartii. Phytopathology 39: 572–579.
Trapero-Casas, A. & R.M., Jiménez-Díaz, 1985. Fungal wilt and root rot diseases of chickpea in Southern Spain. Phytopathology 75: 1146–1151.
Wheeler, H.E. & H.S., Black, 1963. Effects of Helminthosporium victoriae and victorin upon permeability. American Journal of Botany 50: 686–693.
Wheeler, H.E., 1976. Permeability alterations in diseased plants. In: R., Heitefuss & P.H., Williams (Eds). Physiological Plant Pathology. Vol 4, pp. 413–429. Springer-Verlag, New York.
Yoder, O.C., 1980. Toxins in pathogenesis. Annual Review of Phytopathology 18: 103–129.
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Botta, G.L., Dimarco, M.P., Melegari, A.L. et al. Potential of a Fusarium eumartii culture filtrate on the screening for wilting resistance in potato. Euphytica 80, 63–69 (1994). https://doi.org/10.1007/BF00039299
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DOI: https://doi.org/10.1007/BF00039299