Summary
A procedure for plant regeneration from callus culture of potato, Solanum tuberosum L. is described. Calli were induced from 1–2 mm long shoot apices of potato cultivars Cara and A25/19 on half-strength Murashige and Skoog's medium (half-MS) supplemented with 3.2 mg IAA (indole-3-acetic acid), 1.0 mg kinetin (6-furfurylamino)purine], and 0.5 mg 2,4-D [2,4-dichlorophenoxy)acetic acid]/1. Sixty percent explants produced nodular calli on this medium within 30 days. Calli differentiated into shoot-primordia when subcultured on half-MS medium supplemented with 0.5 mg 2,4-D and 1.0 mg zeatin [6-(4-hydroxy-3-methybut-2 enylamino)amino purine]/1. Differentiated calli on half-MS medium without growth hormones produced complete plantlets which were cloned on the same medium and transferred into soil.
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Behnke, M., 1976. Kulturen isolierten Zellen von einigen dihaploiden Solanum tuberosum — Klonen und ihre Regeneration. Z. Pflanzenphysiol. 78: 177–181.
Binding, H., R., Nehls, O., Schieder, S. K., Sopory & G., Wenzel, 1978. Regeneration of protoplasts isolated from dihaploid clones of Solanum tuberosum. Physiol. Plant. 43: 52–54.
Lam, S. L., 1977. Regeneration of plantlets from single cells in potatoes. Amer. Potato J. 54: 575–580.
Melchers, G., M. D., Sacristan & A. A., Holder, 1978. Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res. Commun. 43: 203–218.
Murashige, T. & F., Skoog, 1962. A revised medium for rapid growth and bioassy with tobacco tissue culture. Physiol. Plant. 15: 473–495.
Roca, W. M., N. O., Espinoza, M. R., Roca & J. E., Bryan, 1978. A tissue culture method for the rapid propagation of potatoes. Amer. Potato J. 55: 691–701.
Roest, S. & G. S., Bokelmann, 1970. In vitro adventitious bud technique for vegetative propagation and mutation breeding of potato (Solanum tuberosum L.). 1. Vegetative propagation in vitro through adventitious shoot formation. Potato Res. 23: 176–181.
Shepard, J. F. & R. E., Totten, 1977. Mesophyll cell protoplasts of potato. Plant Physiol. 60: 313–316.
Shervin, R. M., S. L., Lam & J., Janick, 1975. Plantlet formation from potato callus in vitro. Hort. Sci. 10: 413.
Wang, P. G. & L. C., Huang, 1975. Callus culture from potato tissue and the exclusion of potato virus X from plants regenerated from stem tips. Can. J. Bot. 53: 2565–2567.
Westcott, R. J., G. G., Henshaw & W. M., Roca, 1977. Tissue culture storage of potato germplasm: Culture initiation and plant regeneration. Plant Sci. Letters 9: 309–315.
Wenzel, G., O., Schieder, T., Przewozny, S. K., Sopory & G., Melchers, 1979. Comparison of single cell culture derived Solanum tuberosum L. plants and a model for their application in breeding programs. Theor. Appl. Genet. 55: 49–55.
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Ahloowalia, B.S. Plant regeneration from callus culture in potato. Euphytica 31, 755–759 (1982). https://doi.org/10.1007/BF00039214
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DOI: https://doi.org/10.1007/BF00039214