Abstract
Vacuoles isolated from cultured rose-cell protoplasts by controlled lysis were subjected to partition in polyethylene glycol-dextran two-phase systems. The vacuoles showed a strong affinity for the polyethylene glycol phase, like plasma membrane and unlike other membraneous organelles. After phase partition at 4°C, dilution of the polyethylene glycol destroyed a large proportion of the vacuoles. After phase partition at room temperature (ca. 20°C), the vacuoles in the polyethylene glycol phase were relatively stable to dilution. Two-phase partition may be useful for purification of intact vacuoles and possibly of tonoplast membranes. Minor proportions of acid phosphatase and α-mannosidase were associated with these vacuoles.
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Murphy, T.M., Widell, S. & Hellergren, J. On the use of phase partition to isolate vacuoles from cultured cells. Plant Cell Tiss Organ Cult 7, 205–216 (1986). https://doi.org/10.1007/BF00037737
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DOI: https://doi.org/10.1007/BF00037737