Abstract
A procedure is described to regenerate shoots and bulbs in vitro with high frequency from shoot tips of garlic and shallot plants using benzyladenine or thidiazuron. Regenerated shoots were induced to form bulbs in Murashige and Skoog medium (1962) containing 5 g l-1 activated charcoal and 120 g l-1 sucrose under a long-day photoperiod. Bulbs formed in vitro were transferred to soil without acclimatization and produced viable plants. This method could be useful to produce low-cost bulbs, which are easy to handle and store until needed.
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Abbreviations
- AC:
-
activated charcoal
- BA:
-
benzyladenine
- IBA:
-
indolebutyric acid
- MS:
-
Murashige & Skoog's (1962) medium
- NAA:
-
naphthaleneacetic acid
- TDZ:
-
thidiazuron
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Mohamed-Yasseen, Y., Splittstoesser, W.E. & Litz, R.E. In vitro shoot proliferation and production of sets from garlic and shallot. Plant Cell Tiss Organ Cult 36, 243–247 (1994). https://doi.org/10.1007/BF00037726
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DOI: https://doi.org/10.1007/BF00037726