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Anther and microspore culture ofLupinus albus in liquid culture medium

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Abstract

Embryo-like structures (ELS) with clearly defined cotyledons and radicles have been regenerated fromLupinus albus L. microspores. ELS induction from microspores in liquid medium was successful, with a maximum of over 3,500 ELS per anther being produced from microspores predominantly at the early binucleate stage of development. Cytological analysis revealed that first pollen grain mitosis occurred in closed buds with maximum ELS production being obtained from buds at the point of first petal emergence. Generally there was a lack of synchronisation of microspores within anthers from the less mature bud germination from ELS has not been observed; recurrent somatic embryogenesis occurred following internal cleavage within the ELS or on the surface of the ELS. Methods of increasing the level of mature ELS capable of germination are under investigation.

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Abbreviations

BA:

6-benzyladenine

24-d :

2,4-dichlorophenoxyacetic acid

NAA:

naphthaleneacetic acid

2iP:

N-isopentyl-adenine

GA3 :

gibberellic acid

S&H:

Schenk & Hildebrandt medium (1972)

M&S:

Murashige & Skoog medium (1962)

N&N:

Nitsch & Nitsch medium (1969)

B5:

Gamborg's B5 medium (1968)

NNB5:

N&N salts plus B5 vitamins

ELS:

Embryo Like Structures

DAPI:

4′, 6-Diamidino-2-Phenylindole

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Authors and Affiliations

  1. Department of Agricultural Botany, School of Plant Sciences, University of Reading, Whiteknights, PO Box 221, RG6 2AS, Reading, Berks, UK

    A. J. Ormerod & P. D. S. Caligari

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  1. A. J. Ormerod
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  2. P. D. S. Caligari
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Ormerod, A.J., Caligari, P.D.S. Anther and microspore culture ofLupinus albus in liquid culture medium. Plant Cell Tiss Organ Cult 36, 227–236 (1994). https://doi.org/10.1007/BF00037724

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  • DOI: https://doi.org/10.1007/BF00037724

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