Abstract
Potato (Solanum tuberosum L.) tubers were mass propagated in a jar fermentor using a two-step method consisting of a shoot multiplication step (phase 1) followed by a tuber induction and development step (phase 2). Tuberization was observed within 2 weeks of phase 2 and the number of tubers did not increase after this culture period. In contrast, total tuber weight continuously increased for at least 10 weeks. Although the number of tubers and the total tuber weight clearly decreased under the lower temperature (17°C), this weight decrease was partially prevented by changing the temperature from 17°C to 25°C after 2 weeks of phase 2. This result indicates that tuber development can be controlled independently of induction. Lower temperatures influenced the localization and size distribution of tubers in the jar fermentor.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akita M & Takayama S (1987) Studies on in vitro tuberization of potato (Solanum tuberosum L.) 2. Tuberization in liquid shaken culture. 1987 Abstr. Japan. Soc. Hort. Sic. Autumn Meeting. 266–267 (in Japanese)
Akita M & Takayama S (1988a) Mass propagation of potato tubers using jar fermentor techniques. Acta Hort. 230: 55–61
Akita M & Takayama S (1988b) Studies of in vitro tuberization of potato (Solanum tuberosum L.) 4. Effects of hormonal conditions on tuberization. 1988 Abstr. Japan. Soc. Hort. Sci. Autumn Meeting. 266–267 (in Japanese)
Chandra R, Dodds JH & Tovar P (1988) In vitro tuberization in potato (Solanum tuberosum L.). Intl. Assoc. Plant Tiss. Cult. Newsl. 55: 10–20
Estrada R, Tovar P & Dodds JH (1986) Induction of in vitro tubers in a broad range of potato genotypes. Plant Cell Tiss. Org. Cult. 7: 3–10
Hussey G & Stacey NJ (1984) Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.). Ann. Bot. 53: 565–578
Levin R & Vasil IK (1989) Progress in reducing the cost of micropropagation. Intl. Assoc. Plant Tiss. Cult. Newsl. 59: 2–12
Miller SA & Lipschutz L (1984) Potato. In: Ammirato PV, Evans DA, Shart WR & Yamada Y (Eds) Handbook of Plant Cell Culture, Vol 3 (pp 291–326). Macmillan Publishing, New York
Muller-Rober BT, Kobmann J, Hannah LC, Willmitzer L & Sonnewald U (1990) One of two different ADP-glucose pyrophosphorylase genes from potato responds strongly to elevated levels of sucrose. Mol. Gen. Genet. 224: 136–146
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Wang PJ & Hu CY (1982) In vitro mass tuberization and virus-free seed potato production in Taiwan. Amer. Potato J. 59: 33–37
Wang PJ & Hu CY (1985) Potato tissue culture and its applications in agricuture. In: Li PH (Ed) Potato Physiology (pp 503–577). Academic Press, Orlando
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Akita, M., Takayama, S. Induction and development of potato tubers in a jar fermentor. Plant Cell Tiss Organ Cult 36, 177–182 (1994). https://doi.org/10.1007/BF00037717
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00037717