Abstract
Juvenile leaves of Cupressus arizonica Green (3–5 mm in length) from eight week old seedlings, were cultured on liquid medium supplemented with isopentenyladenine (2 mgl-1). Buds formed from the explants after three weeks of culture, but further growth occurred only after transfer to half-strength medium without plant growth regulators. Histological analysis at different times of culture, showed an early mitotic activity within transfusion tissue, followed by dedifferentiation of epidermal and mesophyll parenchyma cells at the basal zone of the leaves. The differentiation of vascular nodules always preceded bud formation. The difficulty of conifers to root and grow beyond plantlet stage is discussed.
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Di Paola Lipucci, M., Fossi, D., Tognoni, F. et al. Adventitous bud induction in vitro from juvenile leaves of Cupressus arizonica Green. Plant Cell Tiss Organ Cult 10, 3–10 (1987). https://doi.org/10.1007/BF00037491
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DOI: https://doi.org/10.1007/BF00037491