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In vitro culture of tissues, cells and protoplasts of Trifolium alexandrinum L. (Egyptian clover)

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Summary

Callus induction derived from root, hypocotyl and cotyledon explants were investigated for four cultivars of Trifolium alexandrinum L. The Murashige & Skoog (1962) medium containing 2.0 mg/L NAA and 0.5 mg/L BAP was able to induce callus from different explants. A wide range of culture media were tested to determine the morphogenetic potential of different callus types derived from different explant types. Shoot morphogenetic development was observed with the cultivars Sakha 4 and Giza 10. Cell suspension cultures were established from hypocotyl derived callus. Methods for isolation and culture of protoplasts from cotyledons are described.

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Authors and Affiliations

  1. Tissue Culture Laboratory, Department of Crop Science, Faculty of Agriculture, University of Alexandria, Egypt

    M. N. Barakat

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  1. M. N. Barakat
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Barakat, M.N. In vitro culture of tissues, cells and protoplasts of Trifolium alexandrinum L. (Egyptian clover). Euphytica 48, 103–110 (1990). https://doi.org/10.1007/BF00037187

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  • DOI: https://doi.org/10.1007/BF00037187

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