Abstract
The placement of Pinus ponderosa embryos on a benzyladenine (BA) supplemented medium induces the differentiation of two distinct morphological types of cotyledons. Cotyledons in contact with the medium (lower cotyledons) do not elongate, and rapidly respond to BA by forming multiple buds along their entire length. Cotyledons not in contact with the medium (upper cotyledons) elongate, do not form buds, and are similar both morphologically and in protein profiles to seedling cotyledons that do not respond to BA. Using sodium dodecyl sulfate polyacrylamide electrophoresis, protein differences between these two cotyledon types can be detected as early as one week. These protein differences include the reduced level of a 52,000 Kilodalton (52 Kd) peptide and the appearance of a 4 Kd peptide in the lower cotyledons. These peptide differences may prove useful as markers for tissue that will respond to BA.
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Ellis, D.D., Judd, R.C. SDS-PAGE analysis of bud-forming cotyledons of Pinus ponderosa . Plant Cell Tiss Organ Cult 11, 57–65 (1987). https://doi.org/10.1007/BF00036576
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DOI: https://doi.org/10.1007/BF00036576