Abstract
Morphogenic responses within the genus Trifolium were investigated by culturing various explants from seedlings of 72 species. Seedlings from 32 species produced callus alone, 40 produced adventitious shoots and/or roots, of which 25 species produced only shoots and 7 species formed only roots. Seedlings within each species also varied in their response to culture. The section of these seedlings most likely to produce adventitious shoots was the original shoot with the remnants of the surrounding hypocotyl and cotyledons, followed by the excised cotyledons themselves.
Inter- and intra-varietal variation was observed in T. repens. Genotypes that produced adventitious buds were selected and crossed. An improvement in the proportion of the population capable of morphogenesis was observed in one cultivar.
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References
Armstead IP (1986) Transformation studies in Lotus corniculatus. MSc Thesis, Wales
Bhojwani SS, Mullins K and Cohen D (1984) Intra-varietal variation for in vitro plant regeneration in the genus Trifolium. Euphytica 33: 915–921
Bingham ET, Hurley LV, Kaatz DM and Saunders JW (1975) Breeding alfalfa which regenerates from callus tissue in culture. Crop Sci 15: 719–721
Cheyne VA and Dale PJ (1980) Shoot tip culture in forage legumes. Plant Sci Lett 19: 303–309
Dale PJ, Fay MF, Pike LS, Woodcock S and Webb KJ (1984) The transfer of new characters to forage legumes. In: Forage Legumes (Ed DJ Thomson), Occasional Symposium No. 16. British Grassland Society. pp. 66–69
Fay MF and Dale PJ (1984) Legume bloat and tannins in Trifolium species. In: Forage Legumes (Ed DJ Thomson), Occasional Symposium No. 16. British Grassland Society. pp. 212–213
Gleba YY and Sytnik KM (1984) Protoplast fusion. Genetic Engineering in Higher Plants (Ed R Shoeman), Monographs on Theoretical and Applied Genetics 8
MAFF Seed Statistics for 1983
Mee SS, Rhodes I, Rushton W and Webb KJ (1984) Genetic Resources. Report of the Welsh Plant Breeding Station for 1983. p. 49
Meijer EGM and Brown DCW (1985) Screening of diploid Medicago sativa germplasm for somatic embryogenesis. Plant Cell Reports 4: 285–288
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Oelck MM and Schieder O (1983) Genotypic differences in some legume species affecting the redifferentiation ability from callus to plants. Z Pflanzenzuchtg 91: 312–321
Webb KJ, Fay MF, Pike LS, Woodcock S and Dale PJ (1984) Selection of responsive genotypes for protoplast culture in forage legumes. In: Forage Legumes (Ed DJ Thomson), Occasional Symposium No. 16. British Grassland Society. pp. 168–169
Webb J, Woodcock S, Pike LS and Dale PJ (1986) Plant regeneration in the forage legumes. In: Plant Tissue Culture and its Agricultural Applications (Eds LAWithers and PGAlderson), Butterworth Scientific, London. pp. 99–104
Webb KJ, Woodcock S and Chamberlain DA (1987) Plant regeneration from protoplasts of Trifolium repens and Lotus corniculatus. J Pl Breed
Williams EG and Latour Gde (1980) The use of embryo culture with transplanted nurse endosperm for the production of interspecific hybrids in pasture legumes. Bot Gaz 141(13): 252–257
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Webb, K.J., Fay, M.F. & Dale, P.J. An investigation of morphogenesis within the genus Trifolium . Plant Cell Tiss Organ Cult 11, 37–46 (1987). https://doi.org/10.1007/BF00036574
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DOI: https://doi.org/10.1007/BF00036574