Skip to main content
Log in

Adherence of Agrobacterium tumefaciens to the cells of immature wheat embryos

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Agrobacterium attached to wheat embryos in vitro. This attachment was plasmid independent, and occurred on both wounded and unwounded cell surfaces. The pattern of attachment clearly demonstrated that bacterial attachment to cereal cells follows the same trends observed for dicotyledonous plants. During the inoculation period the bacterial cells attach to the plant cell walls either with lateral or polar orientation. Wounding (mechanical or enzymatic) preferentially promoted adherence of the bacteria at the wound site, however, attachment was not wound dependent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Anand VK & Heberlein GT (1977) Crown gall tumorgenesis in potato tuber tissue. Am J. Bot. 64: 153–158

    Google Scholar 

  2. Lippincott BB, Whatley MH & Lippincott JA (1977) Tumor induction by Agrobacterium involves attachment of the bacterium to a site on the host plant cell wall. Plant. Physiol. 59: 388–390

    Google Scholar 

  3. Glogowski W & Galsky AG (1978) Agrobacterium tumefaciens site attachment as a necessary prerequisite for crown gall tumor formation on potato discs. Plant. Physiol. 61: 1031–1033

    Google Scholar 

  4. Pueppke SG & Benny UK (1981) Induction of tumors on Solanum tuberosum L. by Agrobacterium: quantitative analysis, inhibition by carbohydrates and virulence of selected strains. Physiol. Plant Pathol. 18: 169–179

    Google Scholar 

  5. Bryant JA (1986) The role of host-bacterium interactions in the Agrobacterium-mediated transformation of plant cells. Trends Biotechnol. 4: 131–132

    Google Scholar 

  6. Lippincott BB & Lippincott JA (1969) Bacterial attachment to a specific wound site as an essential stage in tumor initiation by Agrobacterium tumefaciens. J. Bacteriol. 97: 620–628

    Google Scholar 

  7. Lippincott JA & Lippincott BB (1978) Cell walls of crown gall tumors and embryogenic plant tissue lack Agrobacterium adherence sites. Science. 199: 1075–1077

    Google Scholar 

  8. Ohyama K, Pelcher LE, Schaefer A & Fowke LC (1979) In vitro binding of Agrobacterium tumefaciens to plant cells from suspension culture. Plant Physiol. 63: 382–387

    Google Scholar 

  9. Matthysse AG, Holmes KV & Gurlitz RHG (1982) Binding of Agrobacterium tumefaciens to carrot protoplasts. Physiol. Plant Pathol. 20: 27–23

    Google Scholar 

  10. Lippincott JA & Lippincott BB (1980) Microbial adherence to plants. Recept. Recognition Ser. B 6: 377–398

    Google Scholar 

  11. Rao SS, Lippincott BB & Lippincott JA (1982) Agrobacterium adherence involves the pectin portion of the host wall and is sensitive to the degree of methylation. Physiol. Plant. 56: 374–380

    Google Scholar 

  12. Bennett RK (1988) Studies on the development of systems for the genetic transformation of crop plants. M.Sc. thesis, University of Sydney, Sydney, Australia.

    Google Scholar 

  13. Czernilofsky AP, Hain R, Herrera-Estrella I, Lorz H, Goyvaerts E, Baker BJ & Schell J (1986) Fate of selectable marker DNA integrated into the genome of Nicotinia tabacum. DNA 5: 101–103

    Google Scholar 

  14. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbour Laboratory

  15. Sen P, Chatterjee G, Kumar PM & Sen SK (1986) Enhancement of attachment of Agrobacterium tumefaciens to plant cell surface results in increase in genetic transformation. Ind. J. Exp. Biol. 24: 153–155

    Google Scholar 

  16. Draper J, Scott R, Armitage P & Walden R (1988) Plant Genetic Transformation and Gene Expression: A Laboratory Manual. Blackwell Scientific, Oxford

    Google Scholar 

  17. Matthysse AG, Holmes KV & Gurlitz RHG (1981) Elaboration of cellulose fibrils by Agrobacterium tumefaciens during attachment to carrot cells. J. Bacteriol 145: 583–595

    Google Scholar 

  18. Matthysse AG & Gurlitz RHG (1982) Plant cell range for attachment of Agrobacterium tumefaciens to tissue culture cells. Physiol. Plant Pathol. 21: 381–387

    Google Scholar 

  19. Matthysse AG, Wyman PM & Holmes KV (1978) Plasmid-dependent attachment of Agrobacterium tumefaciens to plant tissue cultured cells. Infection Immunity 22: 516–522

    Google Scholar 

  20. Whatley MH, Margot JB, Schell J, Lippincott JA & Lippincott BB (1978) Plasmid and chromosomal determination of Agrobacterium adherence specificity. J. Gen. Microbiology 107: 395–398

    Google Scholar 

  21. Douglas CJ, Halperin W & Nester EW (1982) Agrobacterium tumefaciens mutants affected in attachment to plant cells. J. Bacteriol. 152: 1265–1275

    Google Scholar 

  22. Krens FA, Molendijk L, Wullems GJ & Schilperoort RA (1985) The role of bacterial attachment in transformation of cell-wall-regenerating tobacco protoplasts by Agrobacterium tumefaciens. Planta 166: 300–308

    Google Scholar 

  23. Douglas CJ, Staneloni RJ, Rubin RA & Nester EW (1985) Identification and genetic analysis of an Agrobacterium tumefaciens chromosomal virulence region. J. Bacteriol. 161: 850–860

    Google Scholar 

  24. Binns AN & Thomashow MF (1988) Cell biology of Agrobacterium infection and transformation of plants. Annu. Rev. Microbiology. 42: 575–606

    Google Scholar 

  25. Marton L, Wullems GJ, Molendijk L & Schilperoort RA (1979) In vitro transformation of cultured cells from Nicotinia tabacum by Agrobacterium tumefaciens. Nature 277: 129–131

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mooney, P.A., Goodwin, P.B. Adherence of Agrobacterium tumefaciens to the cells of immature wheat embryos. Plant Cell Tiss Organ Cult 25, 199–208 (1991). https://doi.org/10.1007/BF00036211

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00036211

Key words

Navigation