Abstract
Cultured hypocotyl explants of tomatillo (Physalis ixocarpa Brot.), were evaluated with regard to their morphogenic responses to combinations of benzyladenine (BA, 0–5 μM) with either naphthaleneacetic acid (NAA, 0–50 μM) or 2,4-dichlorophenoxyacetic acid (2,4-D, 0–50 μM). The induction of shoots or roots was dependent on the cytokinin/auxin combination.
Hypocotyl explants failed to form shoots when they were grown on media containing either a cytokinin or an auxin alone. The highest frequency of shoot formation was observed on media containing 12.5–25 μM BA and 5 μM NAA. Likewise the highest frequency of root formation was observed on media supplemented with 1 μM BA and 1 μM NAA. Complete plants were regenerated and transferred to soil, where they reached maturity.
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Ramirez-Malagón, R., Ochoa-Alejo, N. Adventitious shoot formation and plant regeneration from tissues of tomatillo (Physalis ixocarpa Brot.). Plant Cell Tiss Organ Cult 25, 185–188 (1991). https://doi.org/10.1007/BF00036209
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DOI: https://doi.org/10.1007/BF00036209