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Shoot organogenesis and plant regeneration in mulberry (Morus bombycis Koidz): Factors influencing morphogenetic potential in callus cultures

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Abstract

Regeneration of multiple shoots via callus induction and organogenesis was achieved in mulberry (Morus bombycis). Pre-soaked internodal explants in 4.4–8.9 μM benzyladenine (BA) formed callus on Linsmaier and Skoog's medium containing 2,4-dichlorophenoxyacetic acid (9.05 μM), α-naphthaleneacetic acid (2.85 μM) and BA (2.2 μM). Explants soaked for 48 to 72 h in low levels of BA produced loose and nodular callus that showed regeneration ability. Calluses developed adventitious shoot buds within 3–4 weeks on medium containing BA (8.9 μM). Fifteen-week-old calluses developed fewer shoot buds than five-week-old calluses, indicating a decrease in morphogenetic potential with increasing duration of callus cultures. Semi-thin section microscopy was used to evaluate incapability of sustained regeneration. Development of normal shoot bud primordia, due to sub-surface reorganisation, was high in young calluses. The decline in the frequency of shoot bud primordia formation with callus ageing is due to reduced cell division activity in epidermal as well as sub-epidermal layers.

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Authors and Affiliations

  1. Mulberry Breeding and Geneties Section, Central Sericultural Research and Training Institute, 570 008, Mysore, India

    A. K. Jain & R. K. Datta

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  1. A. K. Jain
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  2. R. K. Datta
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Jain, A.K., Datta, R.K. Shoot organogenesis and plant regeneration in mulberry (Morus bombycis Koidz): Factors influencing morphogenetic potential in callus cultures. Plant Cell Tiss Organ Cult 29, 43–50 (1992). https://doi.org/10.1007/BF00036145

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  • DOI: https://doi.org/10.1007/BF00036145

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