Skip to main content
SpringerLink
Log in

Influence of virus and virus-like agents on the development of citrus buds cultured in vitro

  • General Paper
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Tissue culture in vitro was used to determine the effect of six major citrus virus and virus-like agents. Nodal stem segments from inoculated Pineapple sweet orange (Citrus sinensis (L.) Osb.), Mexican lime (C. aurantifolia (Christm.) Swing.) and Arizona Etrog citron 861-Sl (C. medica L.) were cultured in vitro to induce shoots. Some virus and virus-like agents had a marked effect on bud development and further recovery of plantlets. The number and size of the shoots that developed from each bud were affected as a result of infection. The effect depended on the specific virus, the isolate and the host-disease combination. The possible implications of these results are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Altman, A, Goren, R (1974) Growth and dormancy cycles in citrus bud cultures and their hormonal control. Physiol Plant 30: 240–245

    Google Scholar 

  2. Altman, A, Goren, R (1977) Horticultural and physiological aspects of citrus bud culture. Acta Hort 78: 51–60

    Google Scholar 

  3. Altman, A, Goren, R (1978) Development of citrus bud explants in culture. J Am Soc Hort Sci 103(1): 120–123

    Google Scholar 

  4. Bar-Joseph, M, Loebenstein, G, Cohen, J (1976) Comparison of particle characteristics and cytopathology of citrus tristeza virus with other morphologically similar viruses. In: Calavan, EC (Ed) Proc 7th Conf Inter Organ Citrus Virol, pp 39–45. University of California, Riverside

    Google Scholar 

  5. Bove, JM, Vogel, R (Ed) (1980) Description and illustration of virus and virus-like diseases of citrus. SETCO, IFAC, Paris

    Google Scholar 

  6. Childs JFL (Ed) (1968) Indexing procedures for 15 citrus diseases of citrus trees. Agric Handbook No 333. ARS, USDA

  7. Duran-Vila N, Ortega V, Navarro L (in press) Morphogenesis and tissue cultures of three citrus species. Plant Cell Tiss Org Cult

  8. Duran-Vila N, Pina JA, Ballester JF, Juarez J, Roistacher CN, Rivera-Bustamante R, Semancik JS (in press) The citrus exocortis disease: a complex of viroid-RNAs. In: Proc 10th Conf Inter Organ Citrus Virol

  9. Duran-Vila, N, Semancik, JS (1982) Effects of exogenous auxins on tomato tissue infected with the citrus exocortis viroid. Phytopathology 72: 777–781

    Google Scholar 

  10. Earle, ED (1978) Phytotoxin studies with plant cells and protoplasts. In: Thorpe, TA (Ed) Frontiers of plant tissue culture 1978, p. 363–372. The International Association for Plant Tissue Culture, Calgary, Alberta, Canada

    Google Scholar 

  11. Favre, JM, Juncker, B (1987) In vitro growth of buds taken from seedlings and adult plant material in Quercus robur L. Plant Cell Tiss Org Cult 8: 49–60

    Google Scholar 

  12. Giladi, I, Altman, A, Goren, R (1977) Differential effects of sucrose, abscissic acid, and benzyladenine on shoot growth and callus formation in the abscission zone of excised citrus buds. Plant Physiol 59: 1161–1164

    Google Scholar 

  13. Gonsalves, D, Garnsey, SM (1976) Association of particle size with sedimentation velocity of the nucleoprotein components of citrus variegation and citrus leaf rugose viruses. In: Calavan, EC (Ed) Proc 7th Conf Inter Organ Citrus Virol, pp 109–115. University of California, Riverside

    Google Scholar 

  14. Horsch, RB, King, J (1983) A covert contaminant of cultured plant cells: elimination of a Hyphomicrobium sp. from cultures of Datura innoxia (Mill.). Plant Cell Tiss Org Cult 2: 21–28

    Google Scholar 

  15. Kitto, SL, Young, MJ (1981) In vitro propagation of Carrizo citrange. HortScience 16: 305–306

    Google Scholar 

  16. Marton, L, Duran-Vila, N, Lin, JJ, Semancik, JS (1982) Properties of cell cultures containing the citrus exocortis viroid. Virology 122: 229–238

    Google Scholar 

  17. Monette, PL (1986) Micropropagation of kiwifruit using non-axenic shoot tips. Plant Cell Tiss Org Cult 6: 73–82

    Google Scholar 

  18. Murashige, T (1977) Clonal crops through tissue culture. In: Barz, W, Reinhard, E, Zen, MH (Eds) Plant tissue culture and its bio-technological application, p 392–403. Springer-Verlag, Berlin

    Google Scholar 

  19. Murashige, T, Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–497

    Google Scholar 

  20. Navarro L (1981) Effect of citrus exocortis viroid (CEV) on root and callus formation by stem tissue of Etrog citron (Citrus medica L.) cultured in vitro. Proc Int Soc Citriculture, Japan, pp 437–439

  21. Navarro, L, Juárez, J, Ballester, JF, Pina, JA (1980) Elimination of some citrus pathogens producing psorosis-like leaf symptoms by shoot-tip grafting in vitro. In: Calavan, EC, Garnsey, SM, Timmer, LW (Eds) Proc 8th Conf Inter Organ Citrus Virol, pp 162–166. University of California, Riverside

    Google Scholar 

  22. Navarro, L, Roistacher, CN, Murashige, T (1976) Effect of size and source of shoot-tips on psorosis-A and exocortis content of navel orange plants obtained by shoot-tip grafting in vitro. In: Calavan, EC (Ed) Proc 7th Conf Inter Organ Citrus Virol, pp 194–197. University of California, Riverside

    Google Scholar 

  23. Roistacher, CN (1976) Detection of citrus viruses by graft transmission: a review. In: Calavan, EC (Ed) Proc 7th Conf Inter Organ Citrus Virol, pp 175–184 University of California, Riverside

    Google Scholar 

  24. Roistacher, CN, Nauer, EM, Wagner, RL (1980) Transmissibility of cachexia, dweet mottle, psorosis, tatterleaf and infectious variegation viruses on knife blades and its prevention. In: Calavan, EC, Garnsey, SM, Timmer, LW (Eds) Proc 8th Conf Inter Organ Citrus Virol, pp 225–229. University of California, Riverside

    Google Scholar 

  25. Semancik JS, Roistacher CN, Duran-Vila N (in press) Viroid RNA associated with the cachexia (xyloporosis) disease of citrus. In: Proc 10th Conf Inter Organ Citrus Virol

  26. Shepherd, RI, Francki, RIB, Hirth, L, Hollings, M, Inonye, T, Macleod, R, Purcifull, DE, Sinha, RC, Tremaine, JH, Valenta, V, Wetter, C (1976) New groups of plant viruses approved by the international committee on taxonomy of viruses, September 1975. Intervirology 6: 181–184

    Google Scholar 

  27. Tisserat, B (1985) Embryogenesis, organogenesis and plant regeneration. In: Dixon, RA (Ed) Plant cell culture: a practical approach. Oxford: IRL Press, pp 79–105

    Google Scholar 

  28. Tran Thanh Van, KM (1981) Control of morphogenesis in in vitro cultures. Ann Rev Plant Physiol 32: 291–311

    Google Scholar 

  29. Wang, MC, Lin, JJ, Duran-Vila, N, Semancik, JS (1986) Alteration in cell wall composition and structure in viroid-infected cells. Physiol and Molecular Plant Path 28: 107–124

    Google Scholar 

  30. Wallace, JM (1957) Tristeza and seedling yellows of citrus. Plant Dis Rep 41: 394–397

    Google Scholar 

  31. Young, PM, Hutchins, AS, Canfield, ML (1984) Use of antibodies to control bacteria in shoot cultures of woody plants. Plant Sci Lett 34: 203–209

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, Moncada, 46113, Valencia, Spain

    V. Greño, L. Navarro & N. Duran-Vila

Authors
  1. V. Greño
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Navarro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. Duran-Vila
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Greño, V., Navarro, L. & Duran-Vila, N. Influence of virus and virus-like agents on the development of citrus buds cultured in vitro. Plant Cell Tiss Organ Cult 15, 113–124 (1988). https://doi.org/10.1007/BF00035753

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00035753

Key words

Search

Navigation