Abstract
Various explant sources of Populus nigra × P. maximowiczii were used to examine the effects of growth hormones on morphogenesis in vitro. Initial experiments indicated that punctured leaves were superior to non-punctured ones for both callus growth and formation of shoots and roots on MS medium containing various types and concentrations of growth hormones. After 6 weeks in culture, an average of 178 shoots, 129 roots and 3.1 g fresh weight of callus were directly produced from the abaxial side of each punctured leaf. The best combinations of growth hormones for shoot, root and callus proliferation were 0.88 μM BA plus 0.05 μM 2,4-D, 0.44 μM BA plus 2.69 μM NAA and 0.44 μM BA plus 2.26 μM 2,4-D, respectively. Embryoids were also formed on callus derived from punctured leaves. The number of embryoids varied from 0 to 30 per punctured leaf. Adventitious shoots also developed simultaneously with the embryos. Embryoids were removed with a scalpel at the early developmental stages and placed on MS medium lacking growth regulators. Regenerated plantlets were transferred to pots containing vermiculite for normal growth in the greenhouse.
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Park, Y.G., Son, S.H. In vitro organogenesis and somatic embryogenesis from punctured leaf of Populus nigra × P. maximowiczii . Plant Cell Tiss Organ Cult 15, 95–105 (1988). https://doi.org/10.1007/BF00035751
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DOI: https://doi.org/10.1007/BF00035751