Summary
Multiple isoforms of superoxide dismutase (SOD) occurred in grape following an electrophoretic analysis of 75 grapevine accessions. Three activity zones were resolved, and were designated as Sod-1, Sod-2 and Sod-3 in order of their anodal electrophoretic mobility. For the banding pattern and staining intensity, no differences were observed in both ploidy level and somatic mutation. From the controlled cross tests among SOD variants, the grape SOD was presumed as a dimeric enzyme. The grapevine accessions examined exhibited a high level of phenotypic diversity which could be separated into 13 groups on the basis of SOD zymogram. A remarkable difference in SOD phenotypes was recognized between the cultivated varieties and the wild species and rootstocks. Furthermore, we discussed the associations between SOD phenotypes and agronomically important features, especially seedlessness and lime-induced chlorosis.
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Shiraishi, M., Ureshino, K. & Shiraishi, S. Phenotypic polymorphism for superoxide dismutase in grape. Euphytica 89, 249–255 (1996). https://doi.org/10.1007/BF00034613
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DOI: https://doi.org/10.1007/BF00034613